魚針草萃取物抗疲勞作用效應研究

Anti-Fatigue Effect of Aqueous Extract of Anisomelesindica(L.)Kuntze

陳崇實(CHUNG-SHIH CHEN)

指導教授 ： 曾耀銘

朝陽科技大學/理工學院/應用化學系生化科技碩博士班/博士(2016年)

摘要

研究目的:針對台灣民間常用草藥-魚針草進行抗疲勞功效研究。
研究方法:以隨機方式將Balb/c雌鼠分成四組(n=10)，每日投予魚針草萃取物低劑量LA(125mg/kg)、中劑量MA(250mg/kg)、高劑量HA(500mg/kg)、對照組(distilled water)連續28天後，進行強迫游泳試驗，並檢測與疲勞相關的血清生化項目:三酸甘油酯、血糖、乳酸、血胺，並偵測肌肉及肝臟中肝糖濃度。
結果:在研究期間無任何動物死亡，也無任何與劑量相關的副作用發生。由標的器官肝臟與腎臟的病理組織切片中，未發現有意義之損傷。研究期間實驗組與對照組在攝食量及體重無顯著差異。相較於對照組，中、高劑量實驗組在強迫游泳試驗有較持久的游泳時間(p < 0.05)，肌肉或肝臟肝糖濃度也較高(p < 0.05)，三酸甘油酯與血胺濃度也較低(p < 0.05)。此外，實驗組的血糖與乳酸數值雖隨著劑量升高出現改變但未達顯著差異。
結論:本研究是全球首次以動物實驗模式來證實魚針草抗疲勞的功效與相關機轉，也是魚針草抗疲勞治療應用的開端。

關鍵字

肝醣 ； 疲勞 ； 強迫游泳試驗 ； 魚針草 ； 血胺 ； 乳酸

並列摘要

Purpose: To determine the anti-fatigue effect of Anisomeles indica(L.) Kuntze, an herb traditionally used for health improvement in Taiwan.
Methods:Three groups (n = 10 per group) of Balb/c female mice were administered A. indica aqueous extract orally for 28 days at 125 (low dose A. indica, LA), 250 (medium dose A. indica, MA), and 500 (high dose A. indica, HA) mg/kg/day, respectively, while a control group received distilled water. After 28 days, a forced swimming test was performed, and biochemical parameters including plasma triglyceride (TG), glucose, lactate and ammonia levels related to fatigue were examined.
Results: No mice died during the study period. Physical examinations did not reveal any treatment-related adverse effects after dosing, in terms of food and water consumption. Moreover, no obvious peptic ulcers, haemorrhage, or pathological changes in the liver or kidney were observed in the A. indica-treated mice, and there were no significant differences in body weight between the control and treatment groups (p > 0.05). Mice treated with A. indica extract in the MA and HA groups showed a significantly prolonged exhaustive swimming time (p < 0.05), increased hepatic glycogen and muscle glycogen levels (p < 0.05), and decreased triglyceride and plasma ammonia levels (p < 0.05) in a dose-dependent manner, compared with the controls. However, plasma glucose and lactic acid levels were not significantly changed (p > 0.05).
Conclusion:These results provide the first in vivo evidence supporting the anti-fatigue claims associated with A. indicatreatment, indicating that this traditional herb may be of therapeutic use as an ergogenic and anti-fatigue agent.

並列關鍵字

Glycogen ； Fatigue ； Exhaustive swimming test ； Anisomeles indica ； Plasma ammonia ； Lactic acid

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