- 學位論文
常用膳食中草藥對Enterococcusfaecium生長之影響
The Effect of Common Dietary Chinese Herbs on the Growth of Enterococcus faecium
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摘要
本研究探討常用膳食中草藥對乳酸菌生長之影響，並探討其應用於乳酸菌培養之可行性。實驗選用市售之蔘類單方（苦蔘、黨蔘、西洋蔘、高麗蔘、沙蔘、元蔘、丹蔘）、補氣類單方（牛樟芝、木香、砂仁、山楂、枳實、積雪草、黃芩、生薑、厚朴、藿香、佛手、陳皮、紅棗、甘草、白朮、蒼朮、枳殼、巴戟天）以及黃耆搭配各種蔘類之複方膳食中草藥材，利用水萃法進行萃取後，添加於使用羊奶液作為培養基之培養液中進行培養。實驗所測試之菌株為Enterococcus faecium。本研究添加不同膳食中草藥萃取物至菌液中，以觀察菌體生長受影響之情況。 實驗結果顯示，使用羊乳作為培養基並搭配西洋蔘、黨蔘、厚朴中草藥萃取液效果較佳，尤其是以厚朴萃取液表現最好，菌體濃度為無添加中草藥萃取液之菌液的1.8倍(3.6×109CFU/mL)；而黃芩、藿香、紅棗、甘草、蒼朮中草藥萃取液則對Enterococcus faecium有些許抑制的影響。
並列摘要
This research studied the effect of commonly used dietary Chinese herbs on the growth of one of the important lactic acid bacteria, Enterococcus faecium. Water extract of Astragali Radix, Ginseng Radix, Sophorae Radix, Adenophorae Radix, Salivae Miltiorrhizae Radix, Codonopsitis Radix, Scrophulariae Radix and Panacis Quinquefolii Radix, and eighteen other dietary Chinese herbs were added to the liquid culture of E. faecium at various concentration, and cell concentrations were quantified in CFU/mL. Experimental results showed that in 8% w/v goat milk culture medium, the addition of Panacis Quinquefolii Radix, Codonopsitis Radix and Magnolia officinalis extracts exhibited significant growth promotion of up to 1.8 times (3.6×109CFU/mL). Among other tested Chinese herbs, Astragali Radix, Agastache rugosus, Zizyphus jujube, Glycyrrhiza uralensis Fisch and Atractylodes lancea exhibited inhibitory effect on the growth of E. faecium.
參考文獻
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