二苯甲醯基甲烷及其類似物抑制人類子宮頸癌細胞的轉移作用

Dibenzoylmethane and its analogues suppress in vitro metastasis of human HeLa cervical cancer cells

吳宋恩(Sung-en Wu)

指導教授 ： 廖雅芳

朝陽科技大學/理工學院/應用化學系生化科技碩士班/碩士(2013年)

摘要

二苯甲醯基甲烷 (Dibenzoylmethane；DBM) 的化學結構類似於薑黃素 (Curcumin) ，研究指出二苯甲醯基甲烷具有抗腫瘤作用、使細胞週期停滯與造成細胞凋亡等作用。轉移性子宮頸癌及部位未明示子宮癌通常難以治療且在預後情況不理想，若能夠抑制子宮頸腫瘤的轉移將可以有效阻止腫瘤的進展。本研究探討DBM及其類似物包括1-(2-hydroxyphenyl)-3-phenyl-1,3-propanedione (HDB) 和1-(2-hydroxyphenyl-5-methylphenyl)-3-phenyl-1,3-propanedione (HMDB) 對人類子宮頸癌細胞轉移的影響。首先觀察到DBM及其類似物具有濃度依賴性抑制人類HeLa子宮頸癌細胞的存活率。在人類HeLa子宮頸癌細胞的遷移和侵入作用上，DBM及其類似物具有降低此作用的效果。在人類子宮頸癌細胞侵入作用中，第二型基質金屬蛋白酶 (Matrix metalloproteinase-2；MMP-2) 扮演重要的角色。在以DBM及其類似物處理人類HeLa子宮頸癌細胞後，MMP-2的表現量及其活性受到抑制。細胞的貼附性受到E-cadherin/

關鍵字

二苯甲醯基甲烷

並列摘要

Dibenzoylmethane (DBM) is extracted from Glycyrrhiza glabra and its structure is similar to curcumin. It has been shown to exhibit antineoplastic effects, cell cycle arrest and apoptosis. Metastatic cervical cancer often is difficult to treat and a poor prognosis. Therefore, the inhibition of metastasis could prevent tumor progression. In this study, we investigated the effects of DBM and its analogues including 1-(2-Hydroxyphenyl)-3-phenyl-1,3-propanedione (HDB) and 1-(2-Hydroxyphenyl-5-methylphenyl)-3-phenyl-1,3-propanedione (HMDB) on the metastasis of human HeLa cervical cancer cells. DBM and its analogues caused the dose-dependent decrease of cell viability in HeLa cells. Furthermore, they reduced the migration and invasion of HeLa cells. Matrix metalloproteinase-2 (MMP-2) plays the important role in cancer cell invasion. MMP-2 expression and activity were inhibited in DBM-, HDB- and HMDB-treated HeLa cells. Dysfunction or loss of E-cadherin/

並列關鍵字

DBM

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