流道式聚合脢連鎖反應之生物晶片的設計與製作

The design and fabrication of continuous flow polymerase chain reactions biochip

藍培軒(Pai-hsuan Lan)

指導教授 ： 魏哲弘

大同大學/工程學院/機械工程學系所/碩士(2004年)

摘要

近年來聚合脢連鎖反應(Polymerase chain reaction，PCR)已大量應用在DNA複製與生物檢測上，為了縮短反應時間，1998年Kopp提出流道式PCR晶片，將原本3小時的反應時間縮為18分鐘，本研究以流道式PCR晶片為藍本，利用Ansys作熱傳分析，以提供最簡單的製程設計達到其最佳效果，在透過商用PCR實驗中證實兩段溫度PCR為可行後，重新設計兩段溫度的反應機構，藉由減少溫度區簡化結構與控制的變因。製作上，在清洗、鍍膜、蝕刻與晶片結合等製程，我們都分別測試實驗以找出最佳的配方與製程。

關鍵字

連續流道PCR ； 微機電製程

並列摘要

In recent years, polymerase chain reaction (PCR) has been used extensively in DNA duplications and biological examinations. To shorten the reaction time, Kopp in 1998 disclosed a continuous-flow PCR chip to reduce the original reaction time from 3 hours to 18 minutes. This research utilizes the continuous-flow PCR chip for the blueprint and the ANSYS for the thermal conduction analysis to provide the simplest design of the manufacture process and optimize the effect of PCR. After the commercial PCR experiments have showed the feasibility of a dual temperature PCR, the dual temperature reaction mechanism is redesigned as to reduce the number of temperature zones and simplify the structural and control variables. For the manufacturing processes such as rinsing, coating, etching, and chip integration, we conducted experiments and tests to find the best recipe and manufacturing process for the production.

並列關鍵字

MEMS ； polymerase chain reaction

參考文獻

[1] M. A. Northrup, M. T. Ching, R. M.White, R. T. Watson. “DNA amplification with a microfabricated reactionchamber. ”Transducer ’93 _ The 7th International Conference on Solid-State Sensors and Actuators, Yokohama, Japan, pp. 924-926. 1993

[3] Schabmueller, C.G.J.; Evans, A.G.R.; Brunnschweiler, A.; Ensell, G.J.; Leslie, D.L.; Lee, M.A. ”Closed chamber PCR-chips for DNA amplification. ”, IEE Seminar on , 29 March 2000.

[4] M.U.Kopp, A J. de Mello, and Andreas Man, “Chemical amplification: continuous-flow PCR on a chip,” Science, Vol. 280, pp. 1046-1048, 1998.

[5] Qintao Zhang, Wenhui Wang, Hongshen Zhang, and Yuelin Wang, “Temperature analysis of continuous-flow micro-PCR based on FEA,” Sensors and Actuators B, 82, pp. 75–81, 2000.

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