嗜鹽菌萃取生物高分子PHBV之性質研究 與孔洞基材之製備

The Properties of PHBV Biopolymer Extracted from Haloferax mediterranei and the Manufacture of Porous Scaffolds

黃哲孚(Che-fu Huang)

指導教授 ： 陳志成

大同大學/工程學院/生物工程學系所/碩士(2006年)

摘要

組織工程是一門以組織修復為目的的新興科學，構成組織工程的要素中，提供細胞吸附生長的孔洞材料是不可或缺的角色。應用醱酵技術生產之新穎生物高分子聚羥基丁酯戊酯共聚酯 (PHBV)可應用於組織工程的孔洞基材，供細胞生長之用。本研究中經由丙酮與次氯酸鈉處理醱酵菌體再利用酒精沉澱等步驟，自極端嗜鹽菌Haloferax mediterranei醱酵液中回收到高純度的PHBV高分子。研究發現，不同醱酵時間所生產的PHBV高分子具有相近的分子量分布指標 （polydispersity index）（約在1.69~1.85）、相同的化學結構—紅外線光譜法 （FT-IR）、核磁共振光譜法 （NMR）和熱性質分析 （DSC）結果；並且可以利用加入氯仿與醋酸的溶劑添加法製作出泡綿狀的PHBV孔洞基材。亦發現可藉由鹽析法，利用不同的鹽粒徑大小與鹽重量比例製備出PHBV的多孔洞基材。經由變異數分析（ANOVA）的結果顯示，鹽析時所加入鹽的粒徑大小與添加量對孔洞基材的空泡率有影響，於鹽粒徑變大或添加量增加時均造成基材空泡率上升，但孔洞基材的密度與鹽添加量無明顯關係，而與添加之鹽粒徑大小有關。

關鍵字

聚羥基丁酯戊酯共聚酯 ； 高分子性質 ； 孔洞基材 ； 鹽析法

並列摘要

Tissue engineering itself a subject for tissue repair. New materials and new scaffold fabrication which facilitates cell grows and function normally were main concerns. A novel biopolymer derived from bacteria fermentation—PHBV has already utilized in tissue engineering especially as a porous scaffold for cell growth. In the research, we can fist recover a highly purified PHBV polymer by treating bacteria cell mass with acetone or sodium hypochlorite treatment and follows ethanol precipitation process,. We also discover PHBV polymer derived from different sampling time of identical fermentation possess similar molecular property (polydispersity index raging from 1.69 to 1.85) , chemical structure （FT-IR, NMR） and thermal characteristic （DSC）； Moreover can be further cast into sponge-like porous scaffolds by solvent addition technique of addition acetic acid into chloroform. Furthermore, by salt- leaching technique with applying different salt particle size and salt amount, we can fabricate various porous PHBV scaffolds. According to the ANOVA result, void fraction of porous scaffold can be affected by the addition salt size and salt amount, which goes up with the raising of addition salt size or salt amount, while scaffold density reveals only to affected by the addition salt size rather than salt amount.

並列關鍵字

Poly (hydroxybutyric-co-valeric acid) ； porous scaffold ； polymer properties ； salt leaching

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