以固定化技術改善D型胺基酸氧化酶之穩定度

Stabilization of D-amino acid oxidase via immobilization technology

高緒鈞(Hsu-Chun Kao)

指導教授 ： 官宜靜

大同大學/工程學院/生物工程學系所/碩士(2007年)

摘要

無

關鍵字

D型胺基酸氧化酶 ； 固定化

並列摘要

D-Amino acid oxidase (DAO, EC 1.4.3.3) is a key enzyme used for the production of 7-aminocephalosporanic acid, the precursor for the semi-synthesis of cephalosporin antibiotics. Enzyme immobilization is one of the most important techniques used to improve the thermal and pH inactivation and to lengthen the operational half-life of the enzyme applied. In this study, R. toruloides and T. variabilis DAOs were immobilized onto two supports (magnetic-streptavidin beads (MS beads) or CNBr-activated sepharose beads (CS beads)) and their biochemical properties were examined.
The optimal pHs for all immobilized DAOs were shifted from pH 9.0 to 10.0. In comparison with soluble DAOs, immobilized DAOs showed higher pH stability. The improvement in temperature stability was better observed with immobilization on MS beads than on CS beads. With regard to the oxidative stability against hydrogen peroxide, the immobilized TvDAOs showed an increase by 2 to 3.5-fold while the immobilized RtDAOs had an increase by 12 to 20-fold. All DAOs immobilized on MS beads also showed better improvement in the operational stability than those on CS beads.

並列關鍵字

D-amino acid oxidase ； immobilization

參考文獻

李文華，2005，D型胺基酸氧化酶在大腸桿菌異源表現的最適化，碩士論文，生物工程研究所，大同大學，台北。

張永昆，2006，對Rhodosporidium toruloides D型胺基酸氧化酶過氧化氫耐受性的改善，碩士論文，生物工程研究所，大同大學，台北。

Alonso, J., J. L. Barredo, B. Díze, E. Mellado, F. Salto, J. L. García, and E. Cortés. 1998. D-Amino acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217. Microbiology. 144: 1095-1101.

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