以光纖式表面電漿共振感測器檢測生物小分子

Determination of small biomolecule by fiber-optics surface plasma resonance sensor

潘杰(Chien Pan)

指導教授 ： 林銘澤

大同大學/工程學院/生物工程學系所/碩士(2007年)

摘要

光纖式表面電漿共振 (sarface plasmon resonance, SPR) 感測器能夠快速及定量檢測，因此對於監測生物分子間的作用是一項重要的工具，而且光纖式表面電漿共振感測器擁有許多潛在的優點，因為光纖式感測器可再生且不貴可為拋棄式，因此可在複雜的溶液中進行動力學及篩選分析，而不用害怕重複使用昂貴晶片製成的SPR平台所產生的污垢，此外，微小的感測探針以及感測區使得可應用於小體積樣本的檢測，因此其應用範圍比以前越來越廣泛。
本次研究中我們使用大同大學光電所製備之光纖式表面電漿共振感測器來進行小生物分子檢測之研究；此外，利用奈米金粒子及4-choro-1-naphthol沉澱法來放大SPR訊號，發現以奈米金粒子放大訊號之效果並不顯著，但以4-choro-1-naphthol沉澱法來放大SPR訊號，能使SPR訊號明顯增強，且隨著檢測之biotin-albumin bovine serum (biotin-BSA) 及ochratoxin A-ovalbumin (OTA-OVA) 濃度增加，波長訊號與呈色反應也隨之放大，且兩者也呈現正相關，證明酵素反應還原沉澱放大法可應用於此檢測系統。於此檢測條件下，biotin-BSA之有效檢測範圍為0~10 g/ml，且可檢測biotin之有效範圍為10~20 ng/ml。

關鍵字

光纖式表面電漿共振感測器 ； 生物小分子

並列摘要

Fiber-optic surface plasmon resonance (SPR) can be used as a rapid and quantitative assay, and is also an important tool for monitoring biomolecular interactions. Furthermore, the use of fiber-optic SPR provides a number of potential advantages. Because the fiber optic sensors are reproducible and inexpensive to be disposable, binding kinetics and screening analyses can be performed in complex media without fear of irreversibly fouling expensive chipbased SPR platforms. Additionally, the small size of the fibers optic probe and sensing area make the sensors ideal for studying small volumes of samples. The applications are more and more than before.
In this study, we used the fiber-optic SPR sensor designed and obtained from Graduate Institute of Electro-Optical Engineering in Tatung University to determine the small biomolecules. The nanogold particle and precipitation of 4-choro-1-naphthol were used to amplify the detection signals of SPR. The amplification method using nanogold particle was insignificant, but one using precipitation by reducing 4-choro-1-naphthol was able to enhance significantly the SPR signals. The signal changes of wavelength shift were consistent to the increasing in the concentrations of biotin-conjugated albumin bovine serum (biotin-BSA) and ochratoxin A-conjugated ovalbumin (OTA-OVA). Therefore, these results indicated that the amplification method of reducing precipitation by enzyme was suitable to the fiber-optic SPR detection system. The detection limiting for biotin-BSA was 0~10 g/ml, and 10~20 ng/ml for biotin.

並列關鍵字

fiber-optics surface plasma resonance sensor ； small biomolecule

參考文獻

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