本研究將設計開發一光電倍增管(Photomultiplier Tube,PMT)計數傳輸介面電路,並將本研究應用於往後之ATP生物冷光反應技術(ATP Bioluminescence Technique)之開發。光電倍增管(PMT)為一高靈敏度之光電檢測器,其目的是將光子訊號轉變成電子訊號,本研究之介面電路使用日商濱松株式會社(Hamamatsu)所生產之光電倍增管(PMT),型號為H5773,此型號之光電倍增管將光子訊號轉為電子訊號後,經由後級之放大、比較以及調整脈波電路把訊號轉成可計數之TTL電壓位準方波訊號,頻率約數MHz。 爲了計數此一高頻訊號,本研究借助CPLD在高頻處裡的能力來設計此高頻計數器,並將所有的數位邏輯I.C整合至一顆CPLD IC中。CPLD不僅有著極高的工作頻率,且在抑制雜訊方面有著不錯的效果。本研究之介面電路同時搭配8051系列單晶片,目的在於將計數之資料由晶片上之UART傳輸介面傳至個人電腦端的RS232介面,並把資料顯示在人電腦端上。
The purpose of this study is to develop a Photomultiplier Tube(PMT) counting module and apply to ATP Bioluminescence Technique in the future. The PMT is a high sensitivity photoelectric sensor, and the aim is to convert photons into electrons. In this paper, The Hamamatsu Company’s H5773 PMT was be used. The PMT’s output could be converted into a TTL level signal by the Charge Pulse Conditioner Circuit we designed. The output signal’s frequency is about several MHz. For counting the high frequency signal, we use fast speed CPLD and integrate all of the logical circuit into it. The performance of CPLD not only good at speed, but noise suppression. we also use the 8051 microcontroller to transmit the command and counting value to PC by RS-232.Eventually,we hope for further research in Bioluminescence equipment will be developed.