- 學位論文
多孔性滲透膜運用在表面電漿共振光纖生物微流道感測器之研究
THE STUDY OF MICROFLUIDICS WITH POROUS SEMI-PERMEABLE MEMBRANE STRUCTURE FOR OPTICAL FIBER BIOSENSOR BASED ON SURFACE PLASMON RESONANCE
摘要
表面電漿共振被廣泛的應用在化學及生物的特性和分子量級的交互作用測量上。傳統的表面電漿共振感測技術擁有高的靈敏度、動態變化的即時測量、以及在生物感測上無須標地等優點。光纖本身擁有體積小、成本低、並且和表面電漿共振理論相同架構。而近年來生物感測技術日漸成熟，許多生物檢測系統已被大量應用在各個領域。尤其近日來由於流感病毒的大肆傳染，病毒檢測與防疫問題又再度被提出，其中，即時性就地快速篩選系統為世界各國重要的研究目標。 目前高靈敏度的生物檢測系統，都因為其微流體系統之設計不易而失去了即時性就地檢測的可行性。因此，開發一種體積小、成本低、使用方便及操作簡易之生物微流道，但是以微小體積製作生物微流道之設計較具困難性，且不易控制一致性。 從實驗結果得知在結構特性上為多孔性濾膜，搭配吸收墊材產生毛細現象原理，可使檢測液體流動，並且擁有微流道之功用，整體結構簡單且容易操作。針對DI水、酒精、不同濃度的甘油進行測試，和傳統光纖偵測系統具有更好的重覆性及穩定性，同時也成功驗證可以提升在Biotin-BSA的生物檢測之靈敏度，並且在試劑使用上可微量使用並且不具殘留之特性。從這篇論文中，我們可以能成功的証實，利用微陣列多孔性結構，可以大幅提升生物檢測系統應用於即時性就地快速篩選之可行性與便利性。
並列摘要
The phenomenon of surface plasmon resonance (SPR) is widely applied to the biological and chemical sensing for characterizing and quantifying bio- molecular interactions. The traditional advantages of SPR detective method have high sensitive response, kinetic study in real-time, and label-free for biological sensing. Optical fiber sensor has the characteristics of small, low cost, and the same scheme on conventional SPR technique. It is worthy to develop the optical fiber SPR sensor. Recently the flu season is upon us, so Virus detection and preventive issues to be proposed again. So, the real-time detection is a very important research topic. At present, the microfluidics system design is easily for the feasibility in real-time detection. Therefore, development of a small system, low-cost, and disposable easy to use with simple biosensor microfluidics is important, But the biosensor micro-fluidics design is quite complicated and difficult to control the performance. Using porous membrane with a wicking pad absorber to drive the test solutions by capillary phenomena has been carried out as the microfluidics structure in SPR measurement. Besides, a laser diode operated at 690 nm wavelength as the light source was applied to analyze the SPR responses of DI water, ethanol, and glycerin solutions. The results show that in the case of porous semi-permeable membrane structure for the side-polished fiber SPR sensors possess very good repetitive ability and excellent stability in the SPR responses than the original fiber sensor, and can enhanced the sensitivity for the Biotin-BSA sensing. The porous semi-permeable membrane structure we proposed can reduce the reagent dose in sensing and without any residual in the system, and provides the real-time detection ability and convenience in handling in the measurements.