小葉懸鉤子（Rubus taiwanicola Koidz. & Ohwi）組織培養及其抗氧化能力評估

Tissue culture and the evalution of antioxidant activity of Rubus taiwanicola Koidz. & Ohwi.

張維宸(Wei-chen Chang)

指導教授 ： 何錦玟

大同大學/工程學院/生物工程學系所/碩士(2009年)

摘要

將小葉懸鉤子的葉片、葉柄及莖段培養於添加不同Cytokinin的培養基中，葉片在添加0.5 mg/l BA的MS培養基中，經過40天培養，每一葉片可以獲得平均5.67±0.73個芽；莖段培養在0.3 mg/l BA 的MS培養基中，經過30天培養，每一莖段可以獲得平均4.50 ±0.47個芽，再生芽培養在0.1 mg/l IBA的MS培養基中，經過30天培養，發根率可達到100%。植株出瓶前，先培養在1/3MS基礎鹽類培養基的中，可提升植株出瓶存活率至85 %。小葉懸鉤子葉片、葉柄及節間癒合組織的誘導，在含有NAA的培養基中，癒合組織誘導率皆為100%，但同時添加NAA及BA的培養基會有明顯癒合組織產生。其中葉柄及節間在0.5、1.0 mg/l BA與0.8、1.0 mg/l NAA 相互搭配的MS培養基，有較大量的翠綠色癒合組織產生。在液態懸浮培養，於添加0.5 mg/l BA及0.8 mg/l NAA的MS培養基，經21天培養，細胞可以有11倍以上的增殖比率。
小葉懸鉤子田間植株葉片，測試其DPPH自由基清除能力，結果其水及甲醇萃取物，在系統濃度為100 μg/ml清除率都有93%；系統濃度為20 μg/ml清除率也都有72%以上。其水萃物抗氧化力為所有測試樣品中最高。

關鍵字

小葉懸鉤子 ； 組織培養

並列摘要

Leaves, petioles and stem segments of Rubus taiwanicola Koidz. & Ohwi were cultured on MS basal medium supplemented with different cytokinin. The best proliferated response of adventitious shoots was 5.67±0.73 shoots per leaf when cultured on MS basal medium supplemented with 0.5 mg/l BA for 40 days. The proliferated ratio of shoots for was 4.50 ±0.47 shoots per node segment when node segments were cultured on MS basal medium supplemented with 0.3 mg/l BA for 30 days. The frequency of shoot rooting was 100% when regenerated shoots were cultured on MS basal medium supplemented with 0.1 mg/l IBA for 30 days. Plantlets were pre-cultured on 1/3 MS modified medium promoted the survival percentage of plantlets up to 85%. For callus induction of leaves, petioles and stem nodes, the frequency of callus induction was 100% when three kinds of explant cultured on MS basal medium supplemented with NAA. Lots of callus regenerated from the explant of petioles and stem nodes cultured on MS basal medium supplemented with the combination of 0.5, 1.0 mg/l BA and 0.8, 1.0 mg/l NAA. For cell suspension culture, the multiplication ratio of bismass was 11 times when cell cultured in MS basal medium supplemented with 0.5 mg/l BA and 0.8 mg/l NAA for 21 days.
In vivo plantlets and in vitro plantlets of Rubus taiwanicola Koidz. & Ohwi., and 4 rosaceae plants were evaluated the scavenging free radical effect by DPPH test. Water extract and methanol extract from leaves of In vivo plantlets of Rubus taiwanicola Koidz. & Ohwi. expressed highly antioxidation property up 93% under the concntration was 100 μg/ml. Even the system concentration was only 20 μg/ml, the scavenging free radical effect showed up 72%. The results higher than other four kinds of rosaceae plant.

並列關鍵字

Rubus taiwanicola ； Tissue culture

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