- 學位論文
表面固定幾丁聚醣及硫酸軟骨素於熱塑性聚氨酯不織布及ePTFE膜之研究
Immobilization of Chitosan and Chondroitin-6-sulfate Mulitlayers on Thermoplastic polyurethanes (TPU) Nonwoven or e-PTFE Film by Duplex techniques Plasma Treatment and Graft Polymerization
摘要
幾丁聚醣(Chitosan)為天然生物相容性之陽離子性聚醣類高分 子，具有生物相容性並能抑制細菌之代謝進而抑制細菌成長性能。 硫酸軟骨素為為陰性多醣類，可增進傷口癒合及軟骨修復，是一種生 物相容性良好的材料。本論文以熱塑性聚氨基甲酸酯(Thermoplastic Polyurethane,TPU)及拉伸性鐵氟龍(expanded polytetrafluoroethylene， ePTFE)薄膜為為基材，其表面極為疏水的安定性材料，為改善並提升 其表面親水性與生醫特性，本研究利用低溫電漿處理與光表面接枝聚 合技術於基材表面進行改質，再以幾丁聚醣及軟骨素層接式(LBL)形 成多層生解性聚電解質結構。 透過表面接枝聚合及固定生解性高分子之TPU不織布其表面親水 性親水性明顯增加；藉由幾丁聚醣具有帶正電且抑制細菌成長性能， 與具帶負電及生物相容良好之軟骨素共價鍵結於不織布上。透過細胞 貼附率其硫酸軟骨素的接枝可增進不織布之親水性及生物相容性。期 望也具有抑菌效果，可應用於傷口修復等應用。 透過電漿及接枝聚合丙烯酸使ePTFE表面帶負電性，利用LBL自 我組裝改質方式將帶有正負電荷分子，藉其靜電力相互吸引而組成多 層具電解層多醣膜。其水接觸角觀察可知其改質後表面親水性明顯增 加。由SEM及α-step分析可知隨其幾丁聚醣及硫酸軟骨素量隨電解層增加有效的固定於ePTFE上。期待改質後ePTFE能在生物醫學上之應用。
並列摘要
In the study, the improvement of antibacterial activity, of thermoplastic polyurethane (TPU) nonwoven and expanded polytetrafluoroethylene(ePTFE) developed using surface modification of polyelectrolyte complexes immobilization. The presented work was divided into two parts to study. Part 1 investigated the covalent binding of polyelectrolyte complexes biomaterials via pre-plasma treatment and UV-graft polymerization. The polyelectrolyte complexes of Chitosan(ChI)/Chondroitin-6-Sulfate (C-6-S) was covalently immobilized onto the surface of TPU membranes by poly(acrylic acid) (PAA) graft polymerization.The results observed improving the hydrophilicity, antibacterial activity, and cytocompatibility. Part 2 constructed the chitosan/chondroitin-6-sulfate multilayers immobilized on ePTFE film via layer-by-layer (LBL) self-assembly of technique. The resulting observed ePTFE became hydrophilic and 1 to 5 layers of polyelectrolyte complexes of ChI/C-6-S or C-6-S/ChI were successfully fabricated.The multi-layers biomolecules coating on the ePTFE to enhance application of this kind of material on biomedical.