- 學位論文
固定核酸探針於羧化奈米碳管及其在腸炎弧菌之檢測應用
Immobilization of DNA Probe on Carboxylated Carbon Nanotube for Detection of Vibrio parahaemolyticus
摘要
本實驗目的是想藉由奈米碳管的特性，設計出高穩定性、靈敏度的生物感測器。將其固定在無機的電子感測元件表面，進行表面改質，進而發展出可固定DNA探針等各種具生物辨識力之生物感測器。實驗分別在石英晶體微天秤系統(QCM)及梳型電極系統中分別作測試。當奈米碳管進行羧化 (carboxylation) 反應，再以PEI與GA化學鍵結的方式修飾，即可固定設計的單股核酸探針(ss-DNA)片段。當物質固定於QCM 表面時，藉由QCM 頻率下降來確認物質是否固定於無機基材上，將此改質方式應用於無機基材的梳型電極上，當核酸探針的固定及與樣本雜交的反應時，會增加梳型電極的導電度，使阻抗值下降，來偵測其腸炎弧菌的濃度。由結果可知腸炎弧菌濃度於58.4 ng/ml 到1870 ng/ml，其電阻的變化量為1.52 到3.04 歐姆。
並列摘要
Using the property of carbon nanotubes (CNTs) to design the biosensor that be more stable and sensitive. Surface treated CNTs by grafting on inorganic surface for single strand DNA immobilization. In this study, we use two different systems to test. The ss-DNA can be convalently attached to CNTs by way of activated in acid oxidation conditions then PEI and GA chemistry immobilization. We can check the substance immobilization by the frequency shift using the QCM to detect.We can use the same grafting method to the interdigital electrode. When the DNA probe immobilization and hybridization, the resistance will be decrease. As a result, the concentrations of Vibrio parahaemolyticus change from 58.4 ng/ml to 1870 ng/ml, the resistance variate from 1.52 Ω to 3.04 Ω.