可攜式微生物毒性感測器：不同好氧桿菌對廢水毒性反應與判讀方法之比較

Portable Microbial Biosensor:Different Aerobic Bacillus’Response for Wastewater and the Comparison with Determination of Toxicity

陳冠宏(Guan-Hung Chen)

指導教授 ： 廖峻德

中原大學/工學院/醫學工程研究所/碩士(2002年)

https://doi.org/10.6840/cycu200200478

摘要

本研究之微生物感測系統內建特殊算數程式以檢測廢水毒性，使用枯草桿菌、圓形芽胞桿菌及嗜熱桿菌作為感測系統之生物及電化學反應的感測元件。有毒水樣會抑制微生物對氧的攝取，因此產生的微小電流隨配製毒性劑量而改變。利用氧的消耗量轉換為電化學或電荷轉移效應，以兩者關係來推斷該水樣的毒性。量測之電壓與時間的特性圖，以四種數學計算模式表示而得到特定的抑制率，取與溶氧儀之抑制率最相近的數值，並取偵測時間為最迅速的該計算模式，提供一個具充分運算的內建辨識處理系統。分析結果顯示：在最佳電極試片之重活化時間及微生物的生物活性下，各植菌試片能夠偵測酚的濃度範圍在800-4800mgl-1之間；偵測銅離子的濃度範圍在80-160mgl-1之間，但以圓形芽胞桿菌的表現最為靈敏；枯草桿菌對於鋅醇在低濃度時，則視為無毒性物質，其它兩種菌則可對鋅醇的判讀濃度範圍在80-400mgl-1之間。以微生物感測系統與溶氧儀所得到的值做統計分析，三種植菌試片的相關係數分別為：枯草桿菌0.962、嗜熱桿菌0.945、及圓形芽胞桿菌0.781。

關鍵字

微生物感測系統 ； 抑制率 ； 相關係數. ； 計算模式

並列摘要

In this study, a microbial sensing system (MSS) with a built-in special processing algorithm has been developed for detecting wastewater’s toxicity. Bacillus subtilis, Bacillus stearothermophilus and Bacillus sphaericus were utilized as sensing components of the system to initiate biological and subsequent electrochemical reactions. The infinitesimal currents are correlated with the variation of defined dosages of toxicity on the bacteria-attached electrodes. The addition of toxic substances tends to inhibit oxygen uptake rate of the bacteria, which leads to induce electrochemical or charge transfer effects and therefore their relationship can be correlated with the toxicity of sampling water. The characteristic voltage-time curves are fairly interpreted using four types of calculation methods, with which specific inhibition rates (I%) are thereafter expressed. Using the calculated I% close to the value measured by Oxygen Dissolved Analyzer (ODA) and the shortest response time of such method, an optimized MSS with such built-in processing algorithm is provided. With the optimized reactivation time and bioactivity for the bacteria attached on the electrode, the analytical result has demonstrated that these bacteria-attached electrodes are capable to detect phenol concentration in the range of 800-4800mgl-1, Cu2+ concentration in the range of 80-160mgl-1; however, Bacillus sphaericus-attached electrode exhibits the most sensitive interpretation to Cu2+. The Bacillus subtilis-attached electrode has difficulty to distinguish octanol at low concentrations; nevertheless, the other two electrodes are capable to detect octanol’s concentration in the range of 80-400mgl-1. The correlation coefficients between measurements using MSS and ODA are 0.962 for Bacillus subtilis-, 0.945 for Bacillus stearothermophilus-, 0.781 for Bacillus sphaericus-attached electrode, respectively.

並列關鍵字

Correlation coefficient. ； Inhibition rate ； Calculation method ； Microbial sensing system

參考文獻

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