- 學位論文
小金櫻果實對黑色素生成及膠原蛋白生成之影響
Effects of Rosa Taiwanensis Fruits on th Melanogensis and Collagen Formation
摘要
酪胺酸酶(EC 1.14.18.1)為脊椎動物皮膚中黑色素生成路徑中速率限制步驟之關鍵酵素。對皮膚黑色素細胞兼具低毒性及減少黑色素生成之天然物已成為近年皮膚美白研究之趨勢。本研究探討傳統草藥小金櫻(Rosa taiwanensis)果實之各種溶劑萃取物抑制B16F0老鼠皮膚黑色素細胞黑色素之生成。結果顯示甲醇及70%乙醇萃取物對B16F0細胞存活率顯著較熱水萃取者高；而70%乙醇、熱水萃取物則可顯著的抑制B16F0細胞之黑色素生成及酪胺酸酶活性，二者抑制酪胺酸酶活性之IC50分別為71.3及82.5 μg/ml，同時，此二萃取物並能顯著降低B16F0細胞中酪胺酸酶蛋白質表現量。而於三種萃取物中，70%乙醇萃取物可顯著的促進CCD966SK人類皮膚纖維母細胞細胞增生及膠原蛋白的產生。以上顯示小金櫻果實70%乙醇萃取物對黑色素細胞之毒性低，且可顯著地抑制黑色素生成，並可促進皮膚纖維母細胞增生及提高膠原蛋白生成，具有皮膚美白開發之潛力。
並列摘要
Tyrosinase (EC 1.14.18.1) plays an important role on melanogensis in vertebrate. Natural substances with low toxicity to melanocytes and depigmentation activities has attracted the interest for research. In this study, inhibitory effects of various extracts of Rosa taiwanensis fruits on melanogensis were determined. Results showed that 70% ethanolic and methanolic extracts exhibited higher viabilities of B16F0 cells than that of hot water. 70% Ethanolic and hot water extracts significantly reduced the B16F0 melanocyte’s melanin production. And it inhibited tyrosinase activities with IC50 of 71.3 and 82.5 μg/ml, respectively. Meanwhile, they also decreased the protein expression of tyrosinase in B16F0 cells. On the other hand, 70% ethanolic extract significantly increased CCD966SK human fibroblast’s proliferation and collagen formation. These results suggested that 70% ethanolic extract of Rosa taiwanensis fruits with low toxicity to B16F0 melanocytes and depigmentation activity and fibroblast’s proliferation and collagen formation effects might be useful as skin whitening agents.