六十種中藥材熱水萃出物對痤瘡病原菌之抑菌性

In vitro anti-acne activities of hot water extracts from sixty Chinese herbal medicines

鄭景(Ching-Fong Cheng)

指導教授 ： 李鈴

大同大學/工程學院/生物工程學系所/碩士(2005年)

摘要

Propionibacterium acne、Staphylococcus aureus與Staphylococcus epidermidis為痤瘡病灶中之常見菌株，本研究係針對六十種中藥材之熱水萃出物，進行此三株菌株之抑菌性試驗。由disc diffusion assay 篩選出五倍子、黃芩與黃柏對於P. acne BCRC10723、S. aureus BCRC10451與S. epidermidis BCRC10823有顯著之抑菌效果。五倍子、黃芩與黃柏之熱水萃出物對 P. acne BCRC10723之最小抑菌濃度(minimum inhibitory concentration, MIC)分別為0.3125 mg/ml、0.3125 mg/ml與0.625 mg/ml；對S. aureus BCRC10451之MIC分別為0.625 mg/ml、0.625 mg/ml與2.5 mg/ml；對S. epidermidis BCRC10783之MIC則分別為 0.3125mg/ml、0.625 mg/ml與2.5 mg/ml。五倍子、黃芩與黃柏之熱水萃出物經由n-hexane、ethyl acetate、diethyl ether與1-butanol加以區分後，可初步分離出五倍子之抑菌成分位於ethyl acetate區分，黃芩與黃柏之抑菌成分則於1-butanol區分。

關鍵字

熱水萃出物 ； 青春痘 ； 痤瘡桿菌 ； 五倍子 ； 黃芩 ； 中藥 ； 抑菌性

並列摘要

Propionibacterium acne , Staphylococcus aureus and Staphylococcus epidermidis are the common bacterial strains in the acne lesions. Preliminary screening of hot water extracts of sixty of traditional Chinese herbal medicines for antibacterial activities conducted . Rhus chinensis, Scutellaria baicalensis and Phellodendron amurense with antibacterial activitie of P. acne BCRC10723 , S. aureus BCRC10451 and S. epidermidis BCRC10823 were found by disc diffusion assay, the minimum inhibitory concentrations (MIC) of hot water extracts of R. chinensis, S. baicalensis and P. amurense for P. acne BCRC10723 were 0.3125 mg/ml , 0.3125 mg/ml and 0.625 mg/ml, respectively; For S. aureus BCRC10451, the MICs were 0.625 mg/ml , 0.625 mg/ml and 2.5 mg/ml, respectively; For S. epidermidis BCRC10783, the MICs were 0.3125mg/ml , 0.625 mg/ml and 2.5 mg/ml, respectively . After fractionation of R. chinensis, S. baicalensis and P. amurense hot water extracts with n-hexane, followed by ethyl acetate , diethyl ether and 1 – butanol, ethyl acetate fraction of R. chinensis and 1 - butanol fraction of S. baicalensis and P. amurense showed the primising antibacterial activity.

並列關鍵字

acne ； antibacterial activity ； Chinese herbal medicines ； hot water extracts ； Propionibacterium acne ； Rhus chinensis ； Scutellaria baicalensis

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