仿生矽化包覆酯解酶添加溶劑正己烷生產生質柴油

Biodiesel production using lipase encapsulated in biomimetic silica with n-hexane as solvent

李家綺(Chia-Chi Lee)

指導教授 ： 游吉陽

大同大學/工程學院/生物工程學系所/碩士(2012年)

摘要

本研究係利用矽酸(由tetramethoxysilane水解產生)及polyallylamine進行仿生氧化矽包覆lipase (from Pseudomonas cepacia )，並以包覆後酵素催化大豆油或廢食用油與甲醇進行轉酯化反應。利用反應曲面法及Box-Behnken實驗設計分別探討反應溫度(35 - 50℃)、醇油莫耳比(3：1 - 5：1)及溶劑n-hexane添加量(25 - 75%, w/w)對生質柴油轉化率之影響。大豆油最適化條件為43.6℃、醇油莫耳比4.3：1及n-hexane添加油重之75%；預期與實驗轉化率分別為79%及76%。廢食用油最適化條件則為43.3℃、醇油莫耳比5：1及n-hexane添加油重之38%，預期與實驗轉化率分別68%及67%。以大豆油為油源，儲存於4℃或室溫一個月後，生質柴油轉化率均無下降。

關鍵字

正己烷 ； 反應曲面法 ； 生質柴油 ； 酯解酶 ； 仿生氧化矽 ； 助溶劑

並列摘要

Lipase from Pseudomonas cepacia was encapsulated in biomimetic silica which was mediated by polyallylamine. The encapsulated lipase was applied to biodiesel production with soybean oil or waste cooking oil as feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) , and n-hexane concentration (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6℃, substrate molar ratio 4.3：1, and 75% n-hexane. The predicted and experimental values of biodiesel conversion for soybean oil were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3℃, substrate molar ratio 5：1, and 38% n-hexane. The predicted and experimental values of conversion for waste cooking oil were 68% and 67.2 %, respectively. When the encapsulated lipase was stored at 4℃ or room temperature for 1 month, the conversion remained the same with soybean oil as feedstock.

並列關鍵字

response surface methodology (RSM) ； biodiesel ； lipase ； biomimetic silica ； n-hexane ； solvent

參考文獻

Betancor, L., Luckarift, H.R. 2008. Bioinspired enzyme encapsulation for biocatalysis. Trends in Biotechnology, 26(10), 566-572.

Bisen, P., Sanodiya, B., Thakur, G., Baghel, R., Prasad, G. 2010. Biodiesel production with special emphasis on lipase-catalyzed transesterification. Biotechnology Letters, 32(8), 1019-1030.

Canakci, M., Sanli, H. 2008. Biodiesel production from various feedstocks and their effects on the fuel properties. Journal of Industrial Microbiology and Biotechnology 35(5), 431-441.

Chen, G.C., Kuan, I.C., Hong, J.R., Tsai, B.H., Lee, S.L., Yu, C.Y. 2011a. Activity enhancement and stabilization of lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica. Biotechnology letters 33(3), 525-529.

Chen, H.C., Ju, H.Y., Wu, T.T., Liu, Y.C., Lee, C.C., Chang, C., Chung,