仿生氧化矽包覆脂解酶生產生質柴油之最適化

Optimization of biodiesel production using lipase encapsulated in biomimetic silica

蔡秉宏(Bing-hong Tsai)

指導教授 ： 游吉陽

大同大學/工程學院/生物工程學系所/碩士(2012年)

摘要

本論文係利用矽酸(由tetramethoxysilane水解產生)及polyallylamine進行仿生氧化矽包覆脂解酶(from Pseudomonas cepacia )，並以包覆後脂解酶催化大豆油或廢食用油與甲醇進行轉酯化反應。以反應曲面法和Box-Behnken實驗設計探討反應溫度、受質莫耳比(methanol/oil)、含水量(w/w of oil)對生質柴油含量之影響。大豆油轉酯化之最適化反應條件為反應溫度42.3℃、醇油莫耳比4.14、含水量18.72%；預期生質柴油含量為72.7%，實際值為69.5 ± 0.01%。廢食用油轉酯化反應之最適化條件為溫度44.5℃、醇油莫耳比4.69、含水量10%；預期生質柴油含量為72.25%，實際值為72.3 ± 0.01%。以大豆油為基質，儲存於4℃或室溫18天後，生質柴油含量均無下降

關鍵字

反應曲面法 ； 仿生氧化矽 ； 脂解酶 ； 生質柴油

並列摘要

Lipase from Pseudomonas cepacia was encapsulated in biomimetic silica which was mediated by polyallylamine. The encapsulated lipase was applied to biodiesel production with soybean oil or waste cooking oil as feedstock, using methanol as acyl acceptor. The effects of reaction temperature, substrate molar ratio (methanol/oil), and water content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 42.3℃, substrate molar ratio 4.14, water content 18.72%. The predicted and experimental values of fatty acid methyl ester (FAME) content were 72.7% and 69.5%, respectively. The optimal reaction conditions for waste cooking oil were 44.5℃, substrate molar ratio 4.69, water content 10%. The predicted and experimental values of FAME content were both 72.3%. When the encapsulated lipase was stored at 4℃ or room temperature for 18 d, the FAME content remained the same with soybean oil as feedstock.

並列關鍵字

lipase ； biodiesel ； response surface methodology (RSM) ； biomimetic silica

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