利用葡萄糖苷轉移酶與酵母菌固定顆粒連續催化生產高純度異麥芽寡糖

Continuous Production of High-Content Isomalto-Oligosaccharides by Co-Immobilized Transglucosidase and Yeast Cell

游竣凱(Chun kai Yu)

指導教授 ： 許垤棋

大同大學/工程學院/生物工程學系所/碩士(2012年)

摘要

經由葡萄糖苷轉移酶（TG）的作用，麥芽糖轉變成潘糖、異麥芽糖、和其它含有異麥芽糖基的寡糖，以及大量的葡萄糖和麥芽糖。葡萄糖不但是葡萄糖苷轉移酶反應的副產物，而且葡萄糖會抑制葡萄糖苷轉移酶的活性，因此這種異麥芽寡糖含有60%（乾重）的異麥芽寡糖，其它則為葡萄糖和麥芽糖。

本研究利用海藻酸鈣同時固定TG和酵母菌Pichia heimii當作生物觸媒，催化麥芽糖生產高純度異麥芽寡糖。TG催化麥芽糖產生異麥芽寡糖和葡萄糖，而葡萄糖同時被P. heimii消耗，最後可生產純度76%的異麥芽寡糖。然後再利用固定化”Fermipan”酵母做後繼反應，移除大部分的葡萄糖和麥芽糖，可使純度提升到94%。此方法比傳統製程可提高異麥芽寡糖的產率。與單純酵素的反應比較，酵素和酵母的聯合作用產生更多的四糖和五糖，而且可以提高異麥芽寡糖的產率和純度。

關鍵字

Pichia heimii ； 異麥芽寡糖 ； 葡萄糖苷轉移酶 ； 酵母菌

並列摘要

Through the catalytic reaction of transglucosidase (TG) on maltose, maltose is convented into panose, isomaltose and other isomalto-oligosaccharide (IMO) as well as glucose. During the reaction glucose is a by-product and is a competitive inhibitor against transglucosilaion activity of TG. Therefore, this IMO product consists not only of IMO (60% on a dry weight basis), but also large amounts of glucose and maltose.
In this work, calcium alginate encapsulated TG and Pichia heimii served as biocatalysts. Via the catalytic reaction of TG, maltose is converted into IMO and glucose. And concurrently, glucose is consumed by cells of P. heimii. In this way, an IMO up to 76% of purity was obtained. This IMO syrup was successively fermented with immobilized “Fermipan” yeast and the purity of IMO increased to 94%.
Compared with the reaction using TG alone, the current fermentation of yeast made the production of high purity IMO possible and more tetra- and penta-oligosaccharide to be produced.

並列關鍵字

transglucosidase ； Pichia heimii ； isomalto-oligosaccharides ； yeast

參考文獻

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