腸病毒71型 (Enterovirus 71, EV71) 為一重要之人類致病性病毒,最早於1969年在美國加州發現,此後世界各地開始相繼發生腸病毒形成一股流行。1998年台灣地區爆發腸病毒大流行,此次流行以腸病毒71型為主,根據衛生署統計,報告病例高達十三萬件,確定病例405例,死亡病例78例,死亡的病例都集中在五歲以下孩童。直至目前為止台灣地區每年都有腸病毒流行,且目前並無發現有效的藥物或疫苗可預防或治療腸病毒71型,於是本實驗室嘗試在天然食材中尋找具有抑制腸病毒71型的食材,其中發現蘋果汁之百倍稀釋液具有抑制腸病毒之作用,因此決定進一步分析蘋果汁中抑制腸病毒的成分為何,其中發現蘋果中的水溶性纖維具有良好抑制腸病毒71型的效果。於中和試驗與添加蘋果水溶性纖維對細胞內病毒量的影響中發現,試驗前將蘋果水溶性纖維加入腸病毒71型中和後,蘋果水溶性纖維可有效降低病毒量。將腸病毒71型與蘋果水溶性纖維混合以酒精沈澱處理後,以RT-PCR的方法來來測定病毒,進而發現病毒會與蘋果水溶性纖維之間產生交互作用,影響病毒於細胞內之感染與吸附。由此可知蘋果水溶性纖維確實具有抑制腸病毒71型的效果。
Enterovirus 71 (EV71) is an important human pathogenic virus. EV71 was first isolated in 1969 at California. After that, the Enterovirus were took place in everywhere in the world. In 1998, the Enterovirus (EV71 was the major type) were pandemic in Taiwan. According to the statistical data from Department of Health, it showed that up to 130,000 cases had been reported and the death cases were 78 (the death concentrate in children under 5 years). After 1998, EV71 epidemics occurred almost annually in Taiwan. There are no effective vaccines or medicines to prevent and treat the Enterovirus at present, in our previous study, the EV 71 replication could be inhibited by apple juice at a 1:100 dilution. Further analyze the effective component of apple juice; the apple’s soluble fiber is best one to suppress the EV71 replication. Apple''s soluble fiber can effectively reduce viral titer, on the neutralization test. EV71 and the apple’s soluble fiber show the interaction with coprecipitated analysis. These results indicate that apple''s soluble fiber is effective in the inhibition of EV71 replication.