- 學位論文
利用懸浮和包埋假單胞菌置於批次與順序間歇式反應槽中降解酚之動力研究
Kinetics of Degradation of Phenol by Free and Immobilized Pseudomonas Putida Cells in Batch and Sequcing Batch Reactors
摘要
酚和酚類化合物廣泛用於許多行業，如聚合樹脂生產和煉油。這些化合物在工業廢水和地表水中經常出現。溶劑萃取、活性炭吸附和化學氧化法等方法處理酚通常具有缺點，包括高成本處理費用和產生有害的副產物。在各種可用的方法中，生物降解是對環境友好且成本較低的。首先我們由酚濃度為40 mg/L的懸浮及包埋式批次試驗中找出最適當的溫度及pH值，分別為30℃及pH7，在此條件下求五個動力參數：假單胞菌最大比生長率;半飽和常數Ks; 抑制常數Ki; 生長係數Y; 衰亡係數b。在七組批次試驗中我們設定不同酚的初始濃度及測定假單胞菌初始濃度在127小時內每隔數小時取得酚及假單胞菌樣本以測得其濃度及OD值。在假單胞菌的生長曲線於對數生長期求得假單胞菌的比生長率，七組數據再利用生長率對酚初始濃度作圖，使用Haldane動力模式與假單胞菌的生長率作比較，求出最大比生長率，半飽和常數，抑制常數。七組實驗中假單胞菌生物降解及生長曲線可以求出生長率，當酚濃度被假單胞菌消耗完時可由生長曲線取得衰亡係數，動力參數可作為反應槽動力模式輸入值。
並列摘要
Phenols and phenolic compounds are widely used in many industries, such as polymer resin production and refining. These compounds often occur in industrial wastewater and surface water. Treatment of phenols by solvent extraction, activated carbon adsorption, and chemical oxidation processes often has disadvantages, including high cost of processing and the production of harmful by-products. Among the various methods available, biodegradation is environmentally friendly and less costly. First, we found the most suitable temperature and pH value in the suspension and embedded batch tests with initial phenol concentration of 40 mg/L at 30°C and pH7. Under these conditions, five biokinetic parameters were obtained: maximum specific growth rate of P. putida μmax; half-saturation constant Ks; inhibition constant Ki; growth coefficient Y and decay coefficient b. In the seven batch tests, we set the different initial concentration of phenol and measure the concentration of phenol and P. putida cells during 127 hours to determine their concentration and OD value. In the growth curve of P. putida cells, the specific growth rate of P. putida cells was determined in the logarithmic growth phase, and the growth rate of the seven groups of data was plotted against the initial concentration of phenol. The Haldane kinetic model was fitted to experimental data to determine the maximum specific growth rate (μmax), the half saturation constant (Ks) and the inhibition constant (Ki). In the seven groups of experiments, the biodegradation of phenol and growth curves of P. putida cells can be used to determine the yield coefficient (Y) and decay coefficient (b). The biokinetic parameters can be used as the input values of the kinetic model of sequencing batch bioreactor.
參考文獻
延伸閱讀
- 曾綉雅（2002）。懸浮態與固定化細胞降解酚之速率與質傳效應〔碩士論文，元智大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0009-0112200611315382
- 李冠儀、張書文、賴鈺森、林振興（2013）。移動相中添加β-環糊精在逆相液相層析上分離一些單取代酚類位置異構物。嘉南學報（科技類），(39)，56-62。https://www.airitilibrary.com/Article/Detail?DocID=10234470-201312-201410280023-201410280023-56-62
- 蔡怡婷（2018）。酚在固定生物膜反應槽中吸附、脫附與生物降解之動力模式〔碩士論文，中臺科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0099-2705202017051812
- Tsai, S. Y. (2004). 懸浮態與固定化細胞同時降解酚及水楊酸之動力學 [master's thesis, Yuan Ze University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0009-0112200611351435
- 翁善偉（2012）。Synthesis and Optoelectronic Studies of PHBTT by the FeCl3 oxidation method〔碩士論文，國立中正大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0033-2110201613511626