懸浮態與固定化細胞降解酚之速率與質傳效應

Kinetics and Mass Transfer Effect on Phenol Biodegradation Using Suspended and Immobilized Bacteria

曾綉雅(Hsiu-Ya Tseng)

指導教授 ： 莊瑞鑫

元智大學/工程學院/化學工程與材料科學學系/碩士(2002年)

摘要

無資料

關鍵字

生物降解 ； 懸浮態細胞 ； 固定化細胞 ； 基質抑制

並列摘要

Phenolic compounds were often encountered in industrial effluents. It is a serious pollution problem while discharging the wastewater without treatment. A pseudomonas putida strain capable of degrading phenol in wastewater was utilized to investigate the kinetics of phenol removal and, hopefully, to meet the standard of discharge.
In the suspended cell and immobilized cell systems, the degradation rates of phenol were determined as a function of pH values, temperatures, and phenol concentrations. The experimental results showed that the trends of the effects of environmental factors (temperature, pH value, etc.) on the degradation of phenol were similar for both suspended and immobilized cell systems. Generally speaking, the operation at a temperature higher than 40oC and a pH lower than 5 was unfavorable.
The kinetic experiments of the suspended cell systems at 30℃, pH 6.83 and phenol concentrations between 25 to 600 ppm were conducted and the parameters of Haldane model were evaluated. The intermediate (catechol) could be detected in the immobilized cell systems, but this is not the case in the suspended cell systems as well as in the immobilized cell system at low and high levels of phenol. This implied that the effect of mass transfer was considerable in immobilized cell system. The degradation process involved the chemical reaction and intraparticle mass diffusion in the immobilized cell systems.

並列關鍵字

Biodegradation ； Suspended cell ； Immobilized cell ； Substrate-inhibition

參考文獻

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