以電化學法製備聚乳酸仿骨複合編織物之製程與評估

Processing of a Braided PLA Scaffold by a Electrochemical Method for Bone Tissue Enginery

賴柏任(Po-jen Lai)

指導教授 ： 樓靜文

中臺科技大學/健康科學院/醫學工程暨材料研究所/碩士(2012年)

摘要

生醫陶瓷材料在許多臨床運用上主要有氫氧基磷灰石、β-磷酸三鈣等磷酸鈣鹽類，其中氫氧基磷灰石在骨科材料運用較為普遍，但其大多數氫氧基磷灰石經高溫燒結，而導致在人體中不易降解，影響骨骼的生長。
本研究擬將左旋聚乳酸纖維經過加撚工程製備出具有較佳力學強度之聚乳酸加撚紗，並賦予聚乳酸具有撚曲結構以利於氫氧基磷灰石貼附及細胞貼附，在經由編織工程製成多孔空心圓管，並試著改變圓管孔徑。聚乳酸多孔編織物作表面處理與電化學處理，並改變電化學的處理條件，使氫氧基磷灰石貼附在編織物表面上。之後，浸泡幾丁聚醣/明膠溶液後以UV光進行交聯，使氫氧基磷灰石在編織物表面能更加穩固，並探討不同的材料與製程方式對骨修復作用的影響。
實驗結果顯示，本研究以轉筒轉速10000 r.p.m與撚係數4製備聚乳酸複絲，以65/55之捲取齒輪數/編織齒數所製備出之聚乳酸編織物具有穩定之結構，以0.1 A電化學法處理，以及浸泡1/1之幾丁聚醣(1 wt%)與明膠(5 wt%)溶液後以UV光交聯10 min後，製備出之聚乳酸仿骨複合編織物在體外測試有良好之相容性。

關鍵字

電化學處理 ； 氫氧基磷灰石 ； 編織 ； 聚乳酸

並列摘要

The bio-ceramics materials are calcium phosphate such as hydroxyapatite, and β-tricalcium phosphate. Among all, hydroxyapatite is the most common bone substitute material; nevertheless, the high temperature sintering procedure makes hydroxyapatite slower to degrade in the body. This weakness brings side effects to bone regeneration.
In this study, Poly-L-lactide was twisted as a twisting yarn so as to enhance its mechanical strength. Its twisting structure allows hydroxyapatite and cells to adhere to. The Poly-L-lactide twisting yarn was later braided into the porous hollow tubes with different size of aperture. Surface modification and electrochemistry treatment ensured that hydroxyapatite adhere to surface of the braid more easily. Finally, the porous braid was immersed in the chitosan and gelatin solution which was cross-linked with the UV. This crosslink process reinforced bone-like apatite’s adherence to the braid. Effects of different materials and manufactures on bone regeneration were studied.
As a result show that the PLA multi-filaments was twisted to PLA twist yarn by rotor twister. PLA twist yarn used a 16-spindle braid machine to produce the PLA braids. The electrochemistry treatment was performed by 0.1 A in SBF 1 hour, then it was soaked in solution of chitosan (1 wt%)：gelatin (5wt%) solution = 1：1. The PLA braid was irradiated by UV rays and the time period of the irradiation was 10 min. In vitro, it has biocompatibility and non- Toxicity.

並列關鍵字

electrochemical treatment ； hydroxyapatite ； braid ； Polylactide

參考文獻

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