利用HPLC分析台灣市售紅葡萄酒與花生根、虎杖之白藜蘆醇含量

HPLC analysis od the resveratrol contents from red wines sold in Taiwan, and from peanut root and Polygonum cuspidatum

李瑞峰(JUI FENG LI)

指導教授 ： 張竣維

亞洲大學/資訊學院/生物資訊學系碩士班/碩士(2008年)

摘要

白藜蘆醇（resveratrol）是一種多酚類物質，存在於許多植物及食物中，例如：花生、莓類和葡萄。其中在紅葡萄酒(red wine)中含有高含量的白藜蘆醇。研究報告中也指出白藜蘆醇具有預防心血管疾病、抗氧化、抗發炎及抗癌等之生理活性。

本研究主要是以高效能液相層析儀(high performance liquid chromatography；HPLC)針對台灣市售紅葡萄酒、花生根及虎杖（Polygonum cuspidatum）來分析及測定反式(trans-)白藜蘆醇含量，篩選台灣市售十種不同產區之紅葡萄酒。分析結果發現十種市售紅葡萄酒中反式白藜蘆醇之估量介於0.46±0.04～3.35±0.13 mg/L，其中以澳洲所產之紅葡萄酒反式白藜蘆醇估量最高，含有3.35±0.13 mg/L，其次是法國波爾多所產之紅葡萄酒，其反式白藜蘆醇估量約有3.31±0.00 mg/L。若以順式與反式白藜蘆醇總估量來計算，澳洲紅葡萄酒是高於法國波爾多紅葡萄酒。在花生根及虎杖的反式白藜蘆醇含量分析實驗中發現，每公克花生根之反式白藜蘆醇之估量為 0.12±0.00 mg，每公克虎杖之反式白藜蘆醇之估量為0.59±0.00 mg，虎杖中的反式白藜蘆醇的估量較花生根高。

關鍵字

順式白藜蘆醇、反式白藜蘆醇、紅酒、花生根、虎杖

並列摘要

Resveratrol is a polyphenol compound which is distributed in plants and is present in considerable amounts in the human diet, such as peanut, berries and grapes. The red wine is also found with high content of resveratrol. It is reported that resveratrol have preventative functions for cardiovascular diseases, antioxidative effects, anti-inflammatory and anticancer activities.
This study was aimed to evaluate the trans-resveratrol contents from 10 red wines sold in Taiwan, and from peanut root and Polygonum cuspidatum using high performance liquid chromatography (HPLC). Results indicated that the content of trans-resveratrol was from 0.46±0.04 to 3.35±0.13 mg/L in red wines. The red wine from Australia showed higher resveratrol content (3.35±0.13 mg/L) than the others. Peanut root had 0.12 mg/g trans-resveratrol, and Polygonum cuspidatum had 0.59±0.00 mg/g respectively. According to these results, the Polygonum cuspidatum had higher content of trans-resveratrol than peanut root.

並列關鍵字

cis-resveratrol ； trans-resveratrol ； red wine ； peanut root ； Polygonum cuspidatum

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