本實驗,運用乳化非溶劑相分離法,將甲基纖維素(Methyl Cellulose)與表皮生長因子(EGF)的均勻混合水相溶液,製備成芯 材為表皮生長因子,殼材為甲基纖維素的微膠囊,以達到保護表皮生 長因子活性,防止失活,並於釋放時可展現出應有的藥效,且因均勻 分散於微膠囊內,而達到增加接觸表面積的目的。 本實驗為改善乳化相分離法對於操作條件不一致而造成形狀不規 則,其結構易受變動因素影響而呈現藥物溶出或微膠囊破裂之藥效釋 出速率不均一的缺點,故在非溶劑乳化微包覆步驟後,再以賦型劑PVP K30修飾微膠囊外型,藉由PVP K30良好的粘接能力,極易被吸附在膠 體粒子表面起到保護膠體的作用,所呈現出的成膜性,使微膠囊間不 易粘附,以達到均勻釋出藥效的目的。實驗中對製備出乾燥化的微膠 囊樣品分別以冷凍乾燥法及噴霧乾燥法製程行之。 本實驗對緩釋藥效的探討,由測試數據發現,乳化非溶劑與甲基纖 維素溶液的體積比例,在0.5:1為最佳,為減少包覆材料用量,亦可 降低因非溶劑接觸皮膚後,所造成人體過敏或觸發免疫反應的可能。 對於賦型劑PVP K30的探討,因為PVP K30本身具有優良的成膜性與防 粘接能力,故在實驗中的用量以賦型劑:乳化包覆溶液比例=0.1:1, 於SEM照片中顯示出較佳外型。 本實驗以 NIH/3T3老鼠胚胎纖維母細胞對以冷凍乾燥法製備出的 微膠囊樣品施以細胞增生實驗,結果發現非溶劑極低材料毒性、所製 備微膠囊具有保存表皮生長因子活性,並可完整釋放出藥效的性能。
In this study, using emulsion non-solvent phase separation,to f o r m t h e e n c a p s u l a t i o n E p i d e r m a l G r o w t h F a c t o r i n Microencapsulate Composed of Methyl Cellulose. The microcapsules to achieve the protection of epidermal growth factor activity, to prevent deactivation, and was released,to demonstrate the expected efficacy,and because dispersed in the microcapsules, and to increase contact surface area for the purpose. In this subject,to improve the microcapsules's structure and appearance was used the PVP K30 for good adhesion capacity, can easily be adsorbed on the surface of colloidal particles play the role of protective colloid, which demonstrate the film-forming so easy to adhesion between microcapsules to achieve the purpose of uniform release efficacy.Experiments on the preparation of the dried samples of microcapsules were f o l l o w t h e f r e e z e - d r y i n g a n d s p r a y d r y i n g m e t h o d . In this subject, to purchased the NIH/3T3 mouse embryonic fibroblast cells for non-toxic test,in the resultant,the Emulsion non-solvent can complete to release the medicinal properties.