本研究以虱目魚 (Chanos chanos; Milkfish) 魚鱗為原料,比較魚鱗浸漬鹼液脫皮及酸液脫鈣的效果,並比較添加檸檬酸、鳳梨酵素及胃蛋白酶對抽取魚鱗膠原蛋白抽出率之影響,同時製成膠原蛋白面膜及分析其理化性質。研究結果顯示,虱目魚魚鱗經殺菁處理後的感官評估及L值最高,脫膜率為20.71%。在脫鈣部分,魚鱗經由鹽酸分批浸漬後,其鈣含量和灰分含量較低。虱目魚魚鱗經由脫皮膜、去灰分處理後,可提升膠原蛋白抽出率,而胃蛋白酶加檸檬酸採分批萃取方式,其萃取效果最好,產率為17.37%,膠原蛋白抽出率為73.27%。由感官評估試驗結果得知,魚鱗膠原蛋白萃取液經過脫臭處理後其整體評估明顯提升。在抗氧化方面分子量分佈在5-30kDa的膠原蛋白清除DPPH能力最好,且在螯合亞鐵能力及還原力部分的效果也較佳。將虱目魚魚鱗膠原蛋白液添加 15% 聚乙烯醇作為面膜形成劑,其面膜液之黏稠度最大,成膜厚度最大,及成膜效果最好。經皮膚有效性實驗結果顯示分子量5-30 kDa、30-50 kDa及 >50 kDa的膠原蛋白面膜液皆能增加皮膚的保濕度,且在安定性試驗中,各組面膜液皆無發生相分離的現象,顯示產品具良好的安定性。
The aim of this research was used the waste milkfish scale to extract collagen and develop facial mask product. The collagen derived from milkfish scales were produced by different pretreatment and extracting methods. Basing on the test of L.a.b value, ash content, calcium content, solid content, hydroxyproline content, and sensory evaluation, it was found that blanching, decalcification, extracting with pepsin, and deordorization are the four key points to improve the fish scale collagen yield and quality. In this study the optimal collagen-extracting ratio and solid recovery were 73.27% and 17.37% in respectively. It showed that 5-30kDa molecular weight of collagen has the better scavenging activity of DPPH radical、 reducing power and chelation activity on ferrous ion. When collagen facial mask liquid contains 15% polyvinyl alcohol, it can be observed, has the highest liquid viscosity and film forming ability. The liquid collagen mask have the excellent ability to maintain the moisture of skin, and they were very stable without phase separation during 60 day stability test periods.