GABA茶抗氧化活性及對 LPS 誘發微小膠細胞的保護作用

The antioxidant activity and protective effect against LPS -induced microglia toxicity of GABA tea

張榕珊(CHANG JUNE-SHAN)

指導教授 ： 鄧正賢

亞洲大學/健康學院/保健營養生技學系碩士班/碩士(2011年)

摘要

佳葉龍茶是無氧發酵技術的高含量γ-胺基丁酸 (γ-aminobutyric acid，GABA) 的天然茶葉新製品，定義為每100克的茶葉中含有150毫克的γ-胺基丁酸，GABA為抑制性神經傳導物 (inhibitory neurotransmitter)，證實具有降血壓之藥理活性。
本研究利用微小膠細胞於 LPS 誘發發炎與增殖模式下，探討不同濃度 GABA 茶的保護作用，實驗結果顯示，以LPS刺激誘發微小膠細胞 (LPS組) 造成細胞中Nitric oxide (Nitrate/Nitrite) 的含量明顯增加 (19.15±0.63)，且與對照組 (1.35±0.17) 比較具有統計上的顯著性差異 (p < 0.001)。而同時給予LPS 與不同濃度GABA茶 ( 5、25、50、250、500 ng/mL)，以250、500 ng/mL GABA茶對LPS 所造成的微小膠細胞傷害具顯著保護作用 (p < 0.05)。以LPS為mutigen增殖模式下，GABA具有顯著的抑制作用 (p < 0.01)，不同濃度GABA茶則無任何抑制作用，其機轉值得進一步探討。
抗氧化活性方面，以清除 DPPH自由基、普魯士藍生成量及螯合亞鐵金屬離子能力為主要指標，並定量 GABA 茶在不同萃取液中總多酚、類黃酮、GABA、咖啡因含量，以並線性迴歸探討 GABA 茶活性成分與抗氧化能力之相關性。分析結果顯示 GABA 茶模仿泡茶水萃模式下，清除DPPH 自由基能力的EC50 濃度為 3mg/mL，相當於人工合成之抗氧化劑 BHT 的 60%，其還原力測詴 EC50 濃度為 5mg/mL，相當於人工合成之抗氧化劑 BHT 的 55%，螯合亞鐵金屬離子能力 EC50 濃度 10 mg/mL 為相當於 L-ascorbic acid 的 53%。綜合以上之結果，證明GABA 茶泡茶水萃模式下， 統計顯示不具有顯著之抗氧化力與清除自由基之能力，而其抗氧化活性則與總多酚類含量有正相關性。

關鍵字

佳葉龍茶 ； 類黃酮 ； 總多酚

並列摘要

GABA as a kind of tea contains a high amount of γ-amino butyric acid (γ-aminobutyric acid, GABA) natural tea products. Dr. Tian Jin Chi in the Japanese (1987) study the process of how to preserve fresh ingredients Fresh Tea Leaves, and found to contain high concentrations of Fresh Tea Leaves stored in nitrogen, the fresh content of GABA in Fresh Tea Leaves became multiplied. Because such products are also rich in GABA tea so Japan will be named GABA tea or Gabaron tea (Omori, 1992; Li and Zhang, 1993). GABA tea in order to understand Taiwan's chemical composition and its physiological effects.
Study of the Two phases, The first phase of Taiwan's tea production system GABA tea antioxidant content, and to further assess their antioxidant capacity. The second stage will focus on exploration to take advantage of tiny plastic cell culture, the use and proliferation of LPS-induced inflammation model, study the effect of the protective effect of GABA tea.
The first stage is to understand Taiwan's tea production system of GABA tea and its antioxidant antioxidant, antioxidant activity in order to clear the DPPH radical, Prussian blue formation and ferrous metal ion chelating capacity of the main indicators, and quantitatively different GABA tea extract total polyphenols, flavonoids, GABA, caffeine, and linear regression to explore the active ingredient in GABA tea and antioxidant capacity of the correlation. Analysis showed that GABA tea tea water extracts mimic mode, clear the capacity of DPPH radical EC50 concentration 3mg/mL, equivalent to the synthetic antioxidant BHT 60%, the reducing power test EC50 concentration 5mg/mL, equivalent to the synthetic antioxidant BHT 55%, ferrous metal ion chelating ability EC50 concentration of 10 mg / mL L-ascorbic acid is equivalent to 53%. The results of the above, that GABA tea tea water extraction mode, the statistics show that the antioxidant does not have significant power and free radical scavenging ability, and its antioxidant activity and total polyphenol content is a positive correlation.
Phase II results show that the tiny plastic cells induced by LPS stimulation (LPS group) caused the cells Nitric oxide (Nitrate / Nitrite) were significantly increased (19.15 ± 0.63), and the control group (1.35 ± 0.17) compared with the statistics significant difference on the (p <0.001). While giving the concentration of LPS with different GABA tea (5,25,50,250,500 ng / mL), to 250,500 ng / mL GABA tea on the tiny plastic LPS caused a significant protective effect with cell damage (p <0.05 ). The LPS model for the mutigen proliferation, GABA has a significant inhibition (p <0.01), no tea, no different concentrations of GABA inhibition, the mechanism of worth further exploration

並列關鍵字

GABA TEA

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