In this study, different respiratory rate and supine angle change on the cardiovascular modulated system. Subjects were trained with sound pattern in 4- and 10-second breathing, before experiment. We Analyze the Heart Rate Variability (HRV) among the different breathing rate and the title angle. We found that in 4 seconds breathing, supine and 70 degrees title up make the HRV change significantly. In Time domain: the R-R standard deviation, HR standard deviation, R-R average and HR have the significant differences. In Frequency domain: LF (%), HF (%), LF nu, LH / HF, HF nu are significant differences. These results indicated that, different posture and breathing rate could affect the cardiovascular modulated system.