『白高顆─Pueraria Mirifica』之急性毒性試驗

Assessment of Acute Toxicity of "Kwao Keur"

賴東淵(T. Y. Lai) ； 蔡金川(C. C. Tsai) ； 張永腎(Y. H. Chang) ； 劉重民(C. M. Liu) ； 黃柏憲(P. S. Huang) ； 林宜潔(Y. J. Lin) ； 蔡志仁(C. Y. Chai) ； 康婉儀(W. Y. Kang)

《臺灣中醫醫學雜誌》 1卷2期 (2002/03) Pp. 26-37

摘要

本論文探討泰國葛根─白高顆“Kwao Keur-Pueraria mirifice”對小白鼠之急性毒性評估，採用Litchfield and Wilcoxon 方法，分正常對照組及實驗組(高顆水抽出物劑量：0.0mg/kg、1,000mg/kg、3,000mg/kg、10,000mg/kg)，雌雄各四組。進行口服單劑量之短期試驗，觀察小鼠死亡數目及中毒情形以計算急性毒性之半數致死劑量(LD50)。此外，觀察期結束後摘採血液，檢測血清生化指標。結果：急性毒性試驗中，雌雄四組皆無死亡，其半數致死劑量為每公公大於10.0公克，顯示高顆水抽出物毒性甚小。生化指標探討在肝功能方面：對GOT、GPT的影響稍微升高；腎功能方面：雄性小鼠指數稍許升高，雌性小鼠指數卻有低於正常控制組之表現；代謝方面：在TG影響上雄鼠稍許升高，Cholesterol 及 Glucose 有稍微下降，但都未達統計上之意義。

關鍵字

高顆 ； 急性毒性 ； 半數致死劑量(LD50) ； 生化影響

並列摘要

The evaluation of acute toxicity of “Kwao Keur” on mice was conducted using the method of Litchfield and Wilcoxon. Each sex mice were divided randomly into three experimental and control groups (0.0,1.0,3.0 and 10.0 g/kg). Each dose of the crude extract of “Kwao Keur” and normal saline was respectively administered orally in experimental and control group. All the animals were continuously observed for 72 hours for toxicological responses and mortality, therefore calculating the dose of semi – mortality (LD50). After 72 hrs, all the animals were sacrificed and the sera were used to determine the biochemical functions, including liver functions(GOT,GPT), nephritic functions(BUN, Creatinine and uric acid), metabolic functions ( triglyceride, cholesterol and glucose). As a result, the crude extract of “Kwao Kuer” did not cause any death in mice when administered orally in a dose up 10.0g/kg, meaning that toxicity for “Kwao Keur” is very small (LD50＞10.0g/kg). In addition, “Kwao Keur” also did not produce significant toxicological response and serum biochemical data.

並列關鍵字

Kwao Keur - Pueraria mirifica ； Acute toxicity ； Dose for semi -mortality (LD50) ； Biochemical changes