辣椒素誘發人類大腸癌205細胞珠凋亡机轉

The Effects of Capsaicin on the Apoptosis of Human Colon Cancer Colo 205 Cells

呂旭峰(Hsu-Feng Lu) ； 劉嘉又(Jia-You Liu) ； 呂汶紋(Wen-Wen Lu)

《傳統醫學雜誌》 21卷1期 (2010/10) Pp. 1-12

https://doi.org/10.30038/JTM.201010.0001

摘要

近年來在台灣癌症十大死因中，結腸直腸癌一直高居第三位。辣椒素，為辣椒中主要產生辛辣的成分，許多相關研究指出辣椒素具有抗致癌、抗突變及化學防治(chemoprotective)的活性，能引發多種癌症細胞，如：胃癌細胞、前列腺癌細胞及乳癌細胞的凋亡，並具有抑制血管新生的能力。然而目前以辣椒素用於人類大腸癌研究卻很少。本研究結果發現在外在路徑方面，細胞中Fas、FADD蛋白表現增加，造成Caspase-8及-3被活化。內在路徑方面發現，細胞內的ROS、Ca^(2+)產生增加，進而使粒線體上抗凋亡Bcl-2家族蛋白表現下降，無法繼續抑制促凋亡Bcl-2家族蛋白之活性，最後造成粒線體膜電位改變，粒線體釋放出細胞色素c(Cytochrome c)，活化Caspase-9及-3，造成細胞凋亡。結果證明其具有抗腫瘤功效，是一種具開發潛力的抗癌藥物。

關鍵字

辣椒素 ； 化學防治 ； 細胞色素C

並列摘要

In Taiwan, Colorectal cancer is the third most common cancer in men and women. The spicy ingredient in hot pepper was isolated by Thresh, and was named it capsaicin .According to some studies, Capsaicin is an effective component of nature Chinese herb medicine and with a powerful anti-tumor, against mutation and chemoprotective activity. Capsaicin is capable of inducing apoptosis of cancer cells, including gastric cancer, prostate cancer and breast cancer etc. In addition, Capsaicin also inhibits human angiogenesis. However, there are few studies of the effect of Capsaicin on Colorectal cancer. The extrinsic apoptosis pathway is triggered by Fas ligand (FasL) on account of the amount of Fas and FADD protein elevation. These proteins results in the formation of caspase-8 and caspase-3, which trigger the execution of apoptosis. The percentage of ROS and Ca^(2+) levels are significantly different between Capsaicin treated group and control. On account of the expression of anti-apoptosis Bcl-2 family lower down, anti-apoptosis Bcl-2 was not able to inhibit apoptosis in these examined cells and mitochondria membrane released cytochrome c to cause the activations of Caspase-9, -3 which result in apoptosis. From the above cited, Capsaicin is a strong potential agent for the treatment of Colorectal cancer.

並列關鍵字

Capsaicin ； chemoprotective ； Cytochrome c