Some previous studies of elementary and secondary schools have pointed out the important role of teachers' culture in curriculum reform. The enactment of the Early Childhood Education & Care Curriculum Framework (ECECCF) in 2016 is an important time for the transformation of preschool's curriculum. If we could understand the "teachers' culture" in preschools, it would help us to promote the relevant policies from the government. The aim of this study is to explore teachers' culture in a public preschool. The researcher used qualitative interviews and analyzed the characteristics of teachers' culture. The results show: 1. Teachers try to maintain the original daily practices without any changes. 2. The classes in the preschool work independently and separately without cooperation. 3. Teachers are striving for resources for their own classes.