This study intends to examine the efficacy of a computer-assisted emotion recognition training for children with autism spectrum disorder (ASD). A single-subject reversal design was used. Three 9-10 years-old boys with ASD participated in a 4-week training program. The results showed that (1) the participants have made progress in recognizing four target emotions immediately after the training and the learning effect was partially maintained during the follow-up; and (2) the participants failed to generalize their learning to a different assessment situation. It is suggested that future studies expand the contents of the training software to enhance its richness and complexity as well as to simulate real life situations, in order to foster generalization effect.