- 期刊
加味消遙散，蓮子清心飲，龍膽瀉肝湯，香砂六君子湯，四逆散等對肝癌細胞株體外的作用探討
The Effects of Jia-wei-xiao-yao-san, Lian-zi-qing-xin-yin, Long-dan-xie-gan-tang, Xiang-sha-liu-jun-ji-tang and Si-ni-san on Cell Proliferation of Human Hepatoma Cell Lines
摘要
對肝炎的治療，以病毒性肝炎而言，其目的主要使患者肝臟損傷的癒合或肝細胞於分化再生後所產生的變異細胞之清除。由於病毒性肝炎在肝內易導致肝細胞損傷後的再複製，引起肝細胞基因的改變而產生變異的肝細胞，因而引起肝腫瘤。中醫臨床上對肝炎患者的治療，以整體之考量，依其證型分別施以不同的處方。其目的在於調整患者的生理功能，身體之陰陽平衡，達到清除病毒或對變異細胞之清除，避免造成發炎，對肝細胞的傷害，達到對患者最佳的治療作用。 本實驗選擇一些臨床醫師常用於病毒性肝炎的處方，如加味消遙散，蓮子清心飲，龍膽瀉肝湯，香砂六君子湯，四逆散等。探討在體外對肝癌細胞株之毒殺作用。結果於７２小時加味消遙散與四逆散無任何的毒性作用。而蓮子清心飲，龍膽瀉肝湯，香砂六君子湯等三方劑於處理７２小時後對測試二種肝癌細胞株有顯著的生長抑制及毒殺效應。基於上述結果，可建議採用蓮子清心飲，香砂六君子湯等為慢性肝炎患者的因證選用的首選方。而龍膽瀉肝湯因其為苦寒瀉火的方劑，因而對慢性病毒性肝炎需長期服用藥物的患者，並不建議長期採用。
並列摘要
The major therapeutic strategy for hepatitis virus infection is curring the damage hepatocytes or deminishing the mutant hepatic cell caused by virus infection. Treatment with Chinese herb medicines is considering he whole body’ condition, different prescription according to he different symptoms. The purpose of the Chinese medicine therapy may modulate the balance of physiological function and clean the mutant cells, avoid inflammation. In the present study, the crude extracts of jia-wei-xiao-yao-san, lian-zi-qing-xin-yin, long-dan-xie-gan-tang, xiang-sha-liu-jun-ji-tang and si-ni-san, were used to evaluate their effects on cell proliferation of human hepatoma cell lines. Our results showed that jia-wei-xiao-yao-san and si-ni-san did not affect the proliferation of human hepatoma cells. However, the extracts of lian-zi-qing-xin-yin, long-dan-xie-gan-tang and xiang-sha-liu-jun-ji-tang significantly inhibited the growth and promoted the cell death of the human hepatoma cells. Taken together, our observations suggest that lian-zi-qing-xin-yin and xiang-sha-liu-jun-ji-tang are the better therapeutic choices for patients with chronic hepatitis. Long-dan-xie-gan-tang is used, as anti-inflammatory medicine, is not suit for long-term treatment for chronic hepatitis patients.
延伸閱讀
- 姚智榮（1993）。臺灣民間常用生藥""旱蓮草""與""蒲公英""對各種肝毒性物質所誘發急性肝障害的保護作用之研究〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714450625
- 林秀珍、劉芳淑、盧芬鈴、黃成禹、溫國慶（1993）。溫清飲、三黃瀉心湯及小青龍湯中Geniposide、Berberine及Paeoniflorin成分安定性探討。藥物食品分析，1(1)，49-60。https://www.airitilibrary.com/Article/Detail?DocID=10219498-199301-1-1-49-60-a
- 程維德、吳哲豪、廖炎智、李政育（2015）。探討育生補陽還五湯加方用於治療巴金森氏病的療效研究。中國鍼灸學雜誌，3(1)，18-46。https://doi.org/10.6528/CAMS.2015.0002
- Woon, M. D., Wang, Y. H., Lo, H. W., Chung, D., Liu, T. Z., & Chiou, J. F. (2007). The Effect of Hepatoma-Derived Growth Factor, Bcl-2, and Glutathione on Radiation-Induced Apoptosis in Hepatocellular Carcinoma Cell Lines. 放射治療與腫瘤學, 14(3), 179-188. https://doi.org/10.6316/TRO/200714(3)179
- Kim, Y., Rim, Y. A., Yi, H., Park, N., Park, S. H., & Ju, J. H. (2016). The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells International, 2016(), 248-256-019. https://doi.org/10.1155/2016/1329459