Characterization of Phototrophic Purple Non-sulfur Bacteria Isolated from a Eutrophic Lake in Taiwan

分離自台灣優氧化湖之紫色不含硫光合菌

離傑民(Jimmy Kuo) ； 林秀萍(Hsiu-Ping Lin) ； 劉仲康(Jong-Kang Liu)

《Platax》 2007卷 (2007/12) Pp. 39-47

https://doi.org/10.29926/PLATAX.200712.0004

摘要

紫色不含硫光合菌是一群代謝方式多樣且充滿彈性的微生物，在不同的環境可進行不同之代謝模式：包括光合自營、光合異營、化學異營、及化學自營。由於這類細菌的特殊生理特質，近年來受到科學家高度的重視。它們廣泛地分佈於自然界，包括湖泊、水塘、海岸潟湖、水表淤泥或高濃度有機廢水池等。蓮池潭位於高雄市，是一優養化相當嚴重的人工湖泊，由於日照充足且富含有機質，其生態環境相當適合紫色不含硫光合菌的生長。本研究利用Winogradsky column, 由蓮池潭分離出16株菌種。並利用PCR增幅分離菌株之16S rDNA序列，依據16S rDNA基因序列分析結果，確認篩選出的16株菌種皆爲紫色不含硫光合菌。經山親緣關係比對結果發現，這16株紫色不含硫光合菌分屬Rhodopseudomonas、Rubrivivax及Rhodobacter三群。由菌種的特性得知，這三群菌株皆爲革蘭氏陰性菌且都具有細菌葉綠素a與類胡蘿蔔素。對監的耐受度Rhodobacter爲3%，Rhodopseudomonas及Rubrivivax的菌株則爲1%。

關鍵字

紫色不含硫光合菌 ； 蓮池潭

並列摘要

Purple non-sulfur bacteria are a group of metabolically diverse bacteria that can grow photoautotrophically, photoheterotrophically, chemoheterotrophically, or chemoautotrophically. These bacteria are widely distributed in nature occurring in lakes, water ponds, coastal lagoons or high concentration organic waste lagoons. Lotus Pond, located in northern Kaohsiung City, is a hyper-eutrophied artificial lake. Due to sufficient light and pollution with significant amounts of soluble organic matter, the ecology of this lake is suitable for the growth of purple non-sulfur bacteria. In this study, 16 purple non-sulfur bacteria strains were isolated from the water and mud of Lotus Pond using Winogradsky columns. The 16S rDNA fragments of these strains were amplified by polymerase chain reaction, and their DNA sequences were compared with GenBank database. Phylogenetic analysis of these sequences indicated that these strains belong to the following three groups of purple non-sulfur bacteria: Rhodopseudomonas, Rubrivivax, and Rhodobacter. We found that all isolated strains are Gram negative bacteria containing bacteriochlorophyll a and carotenoid. Salt tolerant studies showed that the strain in Rhodobacter group grows well in 3% NaC1, while both Rhodopseudomonas and Rubrivivax groups grow only in 1% NaC1.

並列關鍵字

Purple non-sulfate photosynthetic bacteria ； Lotus Pond