Protein Expression Profile in Juvenile Zebrafish (Danio rerio) Exposed to PBDE-47

受多溴聯苯醚－47暴露的斑馬幼魚(juvenile zebrafish, Danio rerio)其蛋白質表現的變化

鄭金娥(Jing-O Cheng) ； 鄭英敏(Ying-Ming Cheng) ； 周俊廷(Chun-Ting Chou) ； 蕭玉晨(Yu-Chen Hsiao) ； 柯風溪(Fung-Chi Ko) ； 陳德豪(Te-Hao Chen)

《Platax》 2008卷 (2008/12) Pp. 39-46

https://doi.org/10.29926/PLATAX.200812.0004

摘要

Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants used in a wide variety of commercial and household products, such as electronic devices, building materials, and textiles There are 209 theoretical congeners varied in the number and positioning of bromines in the molecules. Among the 209 congeners, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) is the major congener found in most environmental samples and human tissues. Nevertheless, little is currently known about the effects of exposure to PBDE in aquatic organisms This study used proteomics to examine protein expression profile changes in juvenile zebrafish exposed to sublethal concentrations of BDE-47. Juvenile zebrafish were fed food dosed with BDE 47 or a blank from 20 day post hatch (dph) to 38 dph At 38 dph, fish were sampled for protein expression analyses using two-dimensional gel electrophoresis technique. In total there were 587 protein spots detected, with 13 spots changed significantly in the treatment group (p＜0.05), including 6 induced spots and 7 inhibited spots. Our results show that protein expression profile could be affected by BDE-47 exposure in developing zebrafish.

關鍵字

多溴聯苯醚 ； 斑馬幼魚 ； 二維電泳 ； 蛋白質表現

並列摘要

Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants used in a wide variety of commercial and household products, such as electronic devices, building materials, and textiles There are 209 theoretical congeners varied in the number and positioning of bromines in the molecules. Among the 209 congeners, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) is the major congener found in most environmental samples and human tissues. Nevertheless, little is currently known about the effects of exposure to PBDE in aquatic organisms This study used proteomics to examine protein expression profile changes in juvenile zebrafish exposed to sublethal concentrations of BDE-47. Juvenile zebrafish were fed food dosed with BDE 47 or a blank from 20 day post hatch (dph) to 38 dph At 38 dph, fish were sampled for protein expression analyses using two-dimensional gel electrophoresis technique. In total there were 587 protein spots detected, with 13 spots changed significantly in the treatment group (p＜0.05), including 6 induced spots and 7 inhibited spots. Our results show that protein expression profile could be affected by BDE-47 exposure in developing zebrafish.

並列關鍵字

2,2',4,4'-tetrabromodiphenyl ether BDE-47 ； Zebrafish Danio rerio ； Two-dimensional gel electrophoresis ； Protein expression profile