化學發光法之臨床應用－氣喘病人吐氣時所含一氧化氮濃度之測量

The Clinical Application of Chemiluminesce Method - The Measurement of the Asthmatic Patients' Exhaled Nitric Oxide Concentration

朱家成(Chia-Chen Chu) ； 靳宗玫(Tsung-Mei Chin) ； 王家弘(Jia-Horng Wang)

《呼吸治療》 1卷1期 (2002/01) Pp. 45-60

https://doi.org/10.6269/JRT.2002.1.1.07

摘要

Nitric oxide (NO) can be detected in the exhaled air of humans. Several recent studies have demonstrated that the concentration of exhaled NO is increased in patients with asthma, but reduced after patients are treated with inhaled glucocorticoids. In this thesis, we would like to build up a data bank and a method for measurement of exhaled NO concentrations of asthmatic patients in Taiwan. Exhaled NO determinations were performed via a slow vital capacity method in 59 normal and 82 asthmatic subjects by a chemiluminescence analyzer. The exhaled NO level in the normals was 16.4±6.7 ppb, while the levels in the asthmatics, before and after treated with an inhaled bronchodilator, were 32.1±27.6 and 30.3±11.4 ppb, respectively. Obviously, the exhaled NO concentrations determined in patients with asthma were significantly elevated above those of normal subjects. However, there was no statistically significant change (p ＞ 0.005) in NO contents of asthmatic paitents, before and right after treated with a bronchodilator, no matter what age, sex, or level of exhaled NO. It is known that the difference in exhaled NO of normal subjects and asthmatics derives from a difference in NO concentration present in the lower airway, and reflects the inflammatory microenvironment of the asthmatic airway wall. Thus, the measurement of nitric oxide concentrations in exhaled air may represent a noninvasive measure of lower airway inflammation. And, it will be a useful way of monitoring the anti-inflammatory effects of glucocorticoids and other anti-inflammatory treatments in asthma.

關鍵字

化學發光法 ； 吐氣一氧化氮濃度 ； 氣喘 ； 支氣管擴張劑

並列摘要

Nitric oxide (NO) can be detected in the exhaled air of humans. Several recent studies have demonstrated that the concentration of exhaled NO is increased in patients with asthma, but reduced after patients are treated with inhaled glucocorticoids. In this thesis, we would like to build up a data bank and a method for measurement of exhaled NO concentrations of asthmatic patients in Taiwan. Exhaled NO determinations were performed via a slow vital capacity method in 59 normal and 82 asthmatic subjects by a chemiluminescence analyzer. The exhaled NO level in the normals was 16.4±6.7 ppb, while the levels in the asthmatics, before and after treated with an inhaled bronchodilator, were 32.1±27.6 and 30.3±11.4 ppb, respectively. Obviously, the exhaled NO concentrations determined in patients with asthma were significantly elevated above those of normal subjects. However, there was no statistically significant change (p ＞ 0.005) in NO contents of asthmatic paitents, before and right after treated with a bronchodilator, no matter what age, sex, or level of exhaled NO. It is known that the difference in exhaled NO of normal subjects and asthmatics derives from a difference in NO concentration present in the lower airway, and reflects the inflammatory microenvironment of the asthmatic airway wall. Thus, the measurement of nitric oxide concentrations in exhaled air may represent a noninvasive measure of lower airway inflammation. And, it will be a useful way of monitoring the anti-inflammatory effects of glucocorticoids and other anti-inflammatory treatments in asthma.

並列關鍵字

cehemiluminesce ； exhaled nitric oxide ； asthma