一氧化氮(Nitric oxide)與亞硝酸鹽(nitrite)是人體內重要的分子,因此一氧化氮/亞硝酸鹽感測器在生醫上有著極大的研究發展價值。本論文以一氧化氮/亞硝酸鹽光波導感測膜之研究為重點。將NO probe 1,2-Diaminoanthraquinone (DAQ)摻入pHEMA製作出具有可撓性、高選擇性的一氧化氮/亞硝酸鹽水凝膠感測膜,而此薄膜不會因環境pH值改變而造成影響。之後,以薄膜作為波導,藉此增加光程,提高光吸收度,提升薄膜對於一氧化氮/亞硝酸的感測極限與靈敏度。並結合雷射、光纖、CCD光譜儀完成一氧化氮與亞硝酸鹽量測系統,此系統能夠隨時間感測水溶液中的一氧化氮/亞硝酸鹽,而本論文最後證明光波導感測膜主要反應物為亞硝酸鹽。
Nitric oxide (NO) and nitrite (NO2-) are important molecules in the human body. Therefore, NO / NO2- sensor in the biomedical research and development has an immense value. This thesis aims to do the research on NO / NO2- waveguide sensing film. The NO probe molecule 1,2-diaminoanthraquinone (DAQ) is dispersed in Poly 2-hydroxyethyl methacrylate (poly HEMA), to form a NO / NO2- hydrogel sensing film which is high selectivity and flexibility, and this film does not be affected by acid. Next, taking the film as the waveguide to increasing the light path and absorbance which enhance the sensing limit and sensitivity. We combine the sensing film with lasers, fiber, and CCD spectrometer as a measurement system for monitoring aqueous NO / NO2- real-time. Nitrite is the main reactant with waveguide sensing.