- 期刊
天竺鼠迷路動脈阻塞實驗之信度
Experimental Accuracy of Labyrinthine Artery Obstruction in guinea Pigs
摘要
背景：血液循環障礙是內耳急性病變可能的成因。動物實驗將天竺鼠迷路動脈暫時鉗夾造成內耳暫時缺血的模式可以應用於突發性耳聾的致病機轉及藥物治療的研究。本研究的目的是以微小球心臟灌注法來檢驗這動物模式的實驗信度。 方法：天竺鼠20隻，於麻醉及人工呼吸器輔助下，以手術法露出左側迷路動脈，將之以微小血管天加鉗夾。完成後開胸將微小球溶液灌注入左心室。內微小球之含量。有8隻天竺鼠在迷路動脈鉗夾之前以雷射杜卜勒血流測定器測量血流改變。 結果：20隻天竺鼠，只有2隻（10%）左側耳蝸微小球數較右側稍多，其餘18 隻（90%），則全都是左側（鉗夾側）微小球數目較少。左側與右側之比值小於25%者有4隻（20%），小於50%者共有13隻（65%），小於75%者共有14隻（70%）。多數（6/8）用雷射杜卜勒測定法測得耳蝸血流的變化，其結果與微小球法測得的結果相近。 結論：1)迷路動脈鉗夾造成耳蝸缺血的可信度高。2)雷射杜卜勒血流測定法可以快速判斷是否為有效的血管鉗夾。
並列摘要
Background: Acute inner ear disease is possibly caused by vascular accident. We have used the animal model of inner ear ischemia by temporary clamping of the labyrinthine artery in the study of pathogenesis and treatment of sudden deafness. The purpose of this study is to evaluate the accuracy of this clamping method. Methods: In 20 guinea pigs, the left labyrinthine arteries were exposed surgically and clamped wit hmicroclip. Intracardiac catheterization to the left ventricle was done after thoracotmy to infuse microsphere suspension into general circulation. Bilateral temporal bones were harvested, decalcified, and sliced to count the number the microspheres trapped in the cochlea. Results: In 18 guinea pigs (90%) the number of microspheres was less on the experimental side when comparing with the control side. Only 2 (10%) showed dominant capturing of microspheres on the experimental side. The ratio of microsphere number of the experimental to the control side is below 25% in 4 (20%), below 50% in 13 (65%), and below 75% in 14 (70%). The results of laser Doppler flowmetry was compatible to the microshpere study in 6 of the 8 animals. Conclusion: 1) The experimental accuracy of microclip clamping of the labyrinthine artery is high. 2) Laser Doppler flowmetry can be used to evalate the effectiveness of clamping.
延伸閱讀
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