The article aims to study the literary thoughts Zhao Nanxing (1550-1627) and the content of Li Sao Jing Ding Zhu, his work of the studies of Chuci. Zhao was a leader of the Donglin partisans during the later part of the Ming dynasty (1368-1644). Profound in neo-Confucianism and literature, Zhao tried to shape the integrity of his disciples with the help of literature. Apart from the role of a 'textbook', Li Sao Jing Ding Zhu also demonstrates Zhao's political ambition. This book comprises four parts: the preface, Qu Yuan's biography from Shi Ji, the annotations of Li Sao and the postscript. Because of his emphasis in biographical approach, Zhao has made remarkable achievements in philosophical and literary studies of Chuci. Moreover, some semasiological findings are also observed in the annotations part. Compared with the works of the studies of Chuci by the end of the Ming dynasty and early Qing dynasty (1644-1911), Li Sao Jing Ding Zhu is one of the pioneers which made the research in philosophical, literary and documentological ways.