In the test flow of advanced process, testing under high and low temperature has become an essential issue. Testing variables under high and low temperature often confront with the problem of low yield ratio caused by poor contact. Through observation from probe marks on the bonding pad, this study suggests that both the temperature characteristic curve of the components at the design stage and the factors of testing environments, such as testing devices and machine settings, are main causes to affect the yield ratio of testing when facing the temperature variables. After checking the deformation from components and comparing the effects caused by various factors resulting in low yield ratio, a better solution to modify these factors is proposed to overcome the specific effects from environmental uncertainty to the yield ratio.