透過您的圖書館登入
IP:3.146.255.127
  • 期刊

Rise of [Ca^(2+)]i and Apoptosis Induced by M-3M3FBS in SCM1 Human Gastric Cancer Cells

並列摘要


M-3M3FBS (2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide is a presumed phospholipase C activator which induced Ca^(2+) movement and apoptosis in different cell models. However, the effect of m-3M3FBS on cytosolic free Ca^(2+) concentrations ([Ca^(2+)]i) and apoptosis in SCM1 human gastric cancer cells is unclear. This study explored whether m-3M3FBS elevated basal [Ca^(2+)]i levels in suspended cells by using fura-2 as a Ca^(2+)-sensitive fluorescent dye. M-3M3FBS at concentrations between 5-50 μM increased [Ca^(2+)]i in a concentration-dependent manner. The Ca^(2+) signal was reduced by half by removing extracellular Ca^(2+). M-3M3FBS-induced Ca^(2+) influx was inhibited by nifedipine, econazole, SK&F96365, aristolochic acid, and GF109203X. In Ca^(2+)-free medium, 50 μM m-3M3FBS pretreatment inhibited the [Ca^(2+)]i rise induced by the endoplasmic reticulum Ca^(2+) pump inhibitor thapsigargin. Conversely, pretreatment with thapsigargin partly reduced m-3M3FBS-induced [Ca^(2+)]i rise. Suppression of inositol 1,4,5-trisphosphate production with U73122 did not change m-3M3FBSinduced [Ca^(2+)]i rise. At concentrations between 25 and 50 μM m-3M3FBS killed cells in a concentrationdependent manner. The cytotoxic effect of m-3M3FBS was not reversed by prechelating cytosolic Ca^(2+) with acetoxy-methyl ester of bis-(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA/AM). Annexin V/propidium iodide staining data suggest that m-3M3FBS induced apoptosis at 25 and 50 μM. M-3M3FBS also increased levels of superoxide. Together, in human gastric cancer cells, m-3M3FBS induced a [Ca^(2+)]i rise by inducing phospholipase C-independent Ca^(2+) release from the endoplasmic reticulum and Ca^(2+) entry via protein kinase C-sensitive store-operated Ca^(2+) channels. M-3M3FBS induced cell death that might involve apoptosis via reactive oxygen species production.

並列關鍵字

apoptosis Ca^(2+) m-3M3FBS SCM1

參考文獻


Bae, Y.S.,Lee, T.G.,Park, J.C.,Hur, J.H.,Kim, Y.,Heo, K.,Kwak, J.Y.,Suh, P.G.,Ryu, S.H.(2003).Identification of a compound that directly stimulates phospholipase C activity.Mol. Pharmacol..63,1043-1050.
Boittin, F.X.,Petermann, O.,Hirn, C.,Mittaud, P.,Dorchies, O.M.,Roulet, E.,Ruegg, U.T.(2006).Ca2+ -independent phospholipase A2 enhances store-operated Ca2+ entry in dystrophic skeletal muscle fibers.J. Cell Sci..119,3733-3742.
Boittin, F.X.,Gribi, F.,Serir, K.,Bény, J.L.(2008).Ca2+ -independent PLA2 controls endothelial store-operated Ca2+ entry and vascular tone in intact aorta.Am. J. Physiol. Heart Cir. Physiol..295,H2466-H2474.
Bootman, M.D.,Berridge, M.J.,Roderick, H.L.(2002).Calcium signalling: more messengers, more channels, more complexity.Cur. Biol..12,R563-R565.
Brown, R.A.,Leung, E.,Kankaanranta, H.,Moilanen, E.,Page, C.P.(2012).Effects of heparin and related drugs on neutrophil function.Pulm. Pharmacol. Ther..25,185-192.

被引用紀錄


薛植函(2016)。利用第一原理模擬四元半導體銻磷砷化銦的能帶結構〔碩士論文,國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU201602492
Huang, C. L. (2010). 氮化銦鎵量子井結構發光二極體之光學特性分析以及奈米碳管之拉曼光譜研究 [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00140
Liao, G. H. (2014). 電阻式氧化銦錫感測元件與感測電路之研究 [master's thesis, Chaoyang University of Technology]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0078-2611201410190719
Hu, S. H. (2014). 電阻式氧化銦錫氨氣感測器之製備與感測特性之研究 [master's thesis, Chaoyang University of Technology]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0078-2611201410190824

延伸閱讀