蛇床子以及芍片之成分研究

Studies on the chemical constituents of the fruit of Cnidium monnieri (L.) Cusson and the root of Paeonia lactiflora Pall.

胡宛喬(Wan-Chiao Hu)

指導教授 ： 郭悅雄

國立臺灣大學/理學院/化學研究所/碩士(2006年)

https://doi.org/10.6342/NTU.2006.02388

摘要

蛇床子為繖形科植物--蛇床Cnidium monnieri (L.) Cusson的乾燥成熟果實。本實驗將蛇床子以甲醇浸泡得到之萃取物利用矽膠管柱層析、矽膠薄層層析、高效液相層析及再結晶等方法，做進一步的分離純化。目前鑑定得到44個化合物，概分為三類：
一. 香豆素類 (Couamrins)：34個
二. 色酮類 (Chromones)：8個
三. 芳香族類（Aromatic compounds）： 2個
其中有8個新化合物（其中有一個為新骨架）和2個天然界首次分離得到的化合物。
白芍為毛莨科植物芍藥 (Paeonia lactiflora Pall.，栽培種)的根。本實驗將芍片以甲醇浸泡得到之萃取物利用矽膠管柱層析、矽膠薄層層析、高效液相層析及再結晶等方法，做進一步的分離純化。目前鑑定得到13個化合物，概分為五類：
一. 芳香族類 （Aromatic compounds）：8個
二. 固醇類（Steroids）：1個
三. 三萜類（Triterpenoids）：1個
四. 類黃酮類 (Flavonoids)：1個
五. 其他類 (others)：2個
其中有1個是天然界首次分離得到的化合物。
單離以後之各化合物，在測得紅外線光譜，氫核磁共振圖譜，碳核磁共振圖譜與質譜，經推測為已知化合物，則與文獻值比對確定其結構。若是新化合物，則需進行二維核磁共振圖譜實驗，確定其結構。

關鍵字

蛇床子 ； 芍片

並列摘要

Cnidium monnier is a herb annual. Cnidium monnieri (L.) Cusson is dried ripe fruits of Cnidium monnieri has been used in classical traditional Chinese medicine for several diseases. They distributes over Taiwan and mainland China.
The methanol extract from the Cnidium monnieri (L.) Cusson was concentrated to give residue which was partitioned between ethyl acetate and water. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography and HPLC to afford forty-four compounds. Of these compounds, eight compounds are new and two compound are isolated for the first time from natural sources. The total isolated included thirty-four courmarins, eight chromones, and two aromatic compounds.
Paeonia lactiflora Pall. is a herb pereannial. Its dried roots has several medicinal applications in classical traditional Chinese medicine for supplies blood, controls pain, alleviates sudden onset of disease, subdues hyperactive liver, controls excessive sweating. They distributes over Taiwan and mainland China.
The methanol extract from the roots of Paeonia lactiflora Pall. was concentrated to give residue which was partitioned between ethyl acetate and water. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography, and HPLC to afford thirteen compounds. One of these compounds is isolated for the first time from natural sources. The total isolated included eight aromatic compounds, one steroid, one triterpenoid, one flavonoid, and two others were evaluated with telomerase inhibition.
The structure of known compounds were elucidated by comparison of their spectral data with related literature values. The structure of new compounds were identified by spectroscopic methods including 1D and 2D NMR.

並列關鍵字

Cnidium monnieri (L.) Cusson ； Paeonia lactiflora Pall.

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