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  • 學位論文

乳酸對植物葉綠素螢光及抗氧化物質之影響

Effects of lactic acid on chlorophyll fluorescence and antioxidants in plant

指導教授 : 黃文達
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摘要


傳統上,農田多以化學合成除草劑進行雜草管理。但近年來,對環境友善和人類健康的逐漸重視,再加上雜草出現抗藥性,化學合成除草劑的使用逐漸受到質疑,亟需尋求替代的方案,因而天然產物的發現和發展變得更加重要。乳酸除了作為農藥的添加劑外,也有成為除草劑有效成分的潛力。本研究使用大花咸豐草、五節芒和紅豆的葉圓片做為材料,並施用乳酸,結果發現施用乳酸後,皆會造成三種材料的Fv/Fm、ΦPSII,ΦNPQ下降,表示植物受到損害,且使用光能的效率和光保護能力也因此下降。葉綠素含量的下降以及抗氧化物的上升也能證實乳酸會造成植物藥害。本試驗證實乳酸會造成植物損害,因此具有成為除草劑有效成分潛力,乳酸毒性較壬酸低,未來能著重在增強其毒性以及研究其對植物的作用機制。

並列摘要


In conventional agriculture, synthetic herbicides have been used for weed control. However, in recent years, the increasing emphasis on environmental friendliness and human health, coupled with the emergence of resistance to weeds, the use of chemically synthesized herbicides has been increasingly questioned, and there is an urgent need to find alternative solutions, so the discovery and development of natural products has become more important. Lactic acid, in addition to being an adjuvant to pesticides, has the potential to be an active ingredient in herbicides. This study used leaf discs of Bidens pilosa L. var. radiata Sch, Miscanthus floridulus and Vigna angularis as materials. , latic acid caused a decrease in Fv/Fm, ΦPSII, and ΦNPQ of the three materials, indicating that the plants were damaged. The efficiency of using light energy and photoprotective ability were also reduced. Lactic acid also caused a decrease in chlorophyll content and an increase in antioxidants, indicating that lactic acid can cause phytotoxicity. The toxicity of lactic acid was lower than pelargonic acid. This result confirms that lactic acid causes plant damage and therefore has the potential to be an active ingredient of herbicides. In the future, it can focus on enhancing toxicity and mechanism of action on plants.

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