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研究生: 胡凱崴
Hu, Kai-Wei
論文名稱: 探討蛋白質醣化對阿茲海默症中乙型類澱粉蛋白(Aβ)聚集的影響
Exploring the impact of protein glycation on β-amyloid peptide (Aβ) aggregation in Alzheimer's Disease
指導教授: 杜玲嫻
Tu, Ling-Hsien
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
論文出版年: 2019
畢業學年度: 107
語文別: 中文
論文頁數: 93
中文關鍵詞: 醣化最終產物蛋白質聚集阿茲海默症乙型類澱粉蛋白
英文關鍵詞: Advanced glycation end-products, aggregation, Alzheimer’s Disease, β-amyloid
DOI URL: http://doi.org/10.6345/NTNU201900676
論文種類: 學術論文
相關次數: 點閱:68下載:0
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  • 謝誌 i 摘要 ii Abstract iii 目錄 iv 中英對照表 vi 圖索引 xiv 表索引 xvii 第一章 緒論 1 1-1 類澱粉蛋白與人類疾病的聯繫 1 1-2 類澱粉蛋白的結構與生成機制 3 1-3 乙型類澱粉蛋白(β-amyloid peptide, Aβ) 7 1-4 蛋白質的醣化反應 10 1-5 乙型類澱粉蛋白的醣化及醣化修飾位置選擇 15 1-6 乙型類澱粉蛋白與銅離子鍵結構型及氧化壓力關係 25 1-7 研究動機 27 第二章 實驗材料與流程 29 2-1實驗材料與儀器 29 2-2實驗原理與方法 31 第三章 結果與討論 59 3-1胜肽鑑定 59 3-2利用硫磺素T觀察乙型類澱粉蛋白與其醣化修飾胜肽之聚集情形 63 3-3核誘發(seeding)對乙型類澱粉蛋白與其醣化修飾胜肽之聚集影響 65 3-4乙型類澱粉蛋白與其醣化修飾胜肽間的聚集影響 67 3-5觀察乙型類澱粉蛋白與其醣化修飾胜肽之二級結構變化 69 3-6乙型類澱粉蛋白與其醣化修飾胜肽之聚集情況判定 74 3-7乙型類澱粉蛋白與其醣化修飾胜肽粒徑大小 77 3-8乙型類澱粉蛋白及其醣化修飾胜肽與銅(II)離子的結合能力 82 3-9乙型類澱粉蛋白與其醣化修飾胜肽產生羥基自由基(·OH)含量比較 84 第四章 結論 86 參考資料 87

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