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研究生： 羅泓昇
Luo, Hong-Sheng
論文名稱： 邏輯演繹序列串聯質譜法應用於N-聚醣的結構鑑定
Logically Derived Sequence Tandem Mass Spectrometry for Structural Determination of N-Glycans
指導教授： 倪其焜
Ni, Chi-Kung
陳頌方
Chen, Sung-Fang
口試委員： 倪其焜
Ni, Chi-Kung
陳頌方
Chen, Sung-Fang
林震煌
Lin, Cheng-Huang
口試日期： 2022/07/20
學位類別： 碩士
Master
系所名稱： 化學系
Department of Chemistry
論文出版年： 2022
畢業學年度： 110
語文別： 中文
論文頁數： 222
中文關鍵詞： N-聚醣 、結構 、邏輯演繹序列串聯質譜法 、碰撞誘導解離 、豆子 、肉
英文關鍵詞： N-glycans, structure, logically derived sequence tandem mass spectrometry, collision-induced dissociation, beans, meat
研究方法: 實驗設計法 、 比較研究
DOI URL： http://doi.org/10.6345/NTNU202201234
論文種類： 學術論文
相關次數： 點閱：17  下載：0 
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    • 第一章 緒論 1 1.1 碳水化合物的介紹 1 1.2 N-聚醣 3 1.3 生物合成路徑（biosynthetic pathway ）4 1.4 研究背景及動機 5 第二章 實驗方法 7 2.1 實驗藥品、器材與儀器設備 7 2.1.1 實驗藥品 7 2.1.2 實驗儀器 7 2.2 研究流程 8 2.3 實驗步驟 9 2.3.1 從醣蛋白上釋放N-聚醣 9 2.3.2 酸沉澱法 11 2.3.3 乙醇沉澱法 12 2.3.4 固相萃取法(SPE，Solid-Phase Extraction) 12 2.3.5 粒徑篩析層析法（size exclusion chromatography, SEC） 12 2.3.6 二維層析法（Two-dimensional chromatography） 13 2.3.7 親水性液相層析 14 2.3.8 黑豆中Man5GlcNAc2的分離 21 2.3.9 黑豆中Man6GlcNAc2的分離 25 第三章 邏輯演繹序列串聯質譜法 29 第四章 從黑豆中萃取並鑑定高甘露N-聚醣的結構 32 4.1 黑豆中Man5GlcNAc2的異構物一 33 4.1.1 黑豆中Man5GlcNAc2的異構物二 34 4.1.2 黑豆中Man5GlcNAc2的異構物三 35 4.1.3 黑豆中Man5GlcNAc2的異構物四 36 4.1.4 黑豆中Man6GlcNAc2的異構物一 38 4.1.5 黑豆中Man6GlcNAc2的異構物二 40 4.1.6 黑豆中Man6GlcNAc2的異構物三 41 第五章 透過比對數據庫鑑定N-聚醣 43 5.1 各種豆子中高甘露N-聚醣的比較 43 5.2 不同方法處理的紅豆 48 5.3 各種肉類中高甘露N-聚醣的比較 53 5.4 樣品的LC-MS圖譜和高甘露醣數據庫進行比較 57 5.4.1 各種豆類中的Man5GlcNAc2與數據庫進行比較 58 第六章 結論 107 參考文獻 108 附錄A 112

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