香蕉是台灣的主要盛產水果之一，產量過剩常造成價格崩跌導致農民損失重大。而香蕉加工造成的褐變是目前香蕉加工業需要克服的問題之一。本研究目的是利用香蕉添加不同比例的檸檬酸、抗壞血酸和海藻糖製作低褐變香蕉泥，探討超高壓技術加工和熱處理對低褐變香蕉的影響。研究結果顯示，使用0.5％檸檬酸、0.5％抗壞血酸和10％海藻糖同時應用超高壓技術加工處理(HPP)的多酚氧化酶PPO活性為963（U/mg protein），低於對照組9137（U/mg protein）。經60°C處理後，PPO活性為228（U/mg protein）低於對照組7648（U/mg protein）顯著降低PPO活性，經75°C處理後，所有組別的PPO活性完全失活。色澤方面L*、b*值以60°C處理的香蕉泥最高，亮度及黃色度較高，但加熱後會使總酚及總類黃酮含量大幅減少。整體而言，加入檸檬酸、抗壞血酸和海藻糖同時應用超高壓技術加工對抑制香蕉褐變有顯著的效果。最後將香蕉泥製作成香蕉汁，利用超高壓技術加工處理(HPP)並添加不同比例的複合酸抑制褐變，感官品評結果顯示，雖然整體接受度的最高的是控制組，但色澤不佳，而添加2%抗壞血酸的組別E在各項分數上都較接近控制組，色澤接受度較高，未來在口味上可做調整。添加2%抗壞血酸在貯藏期間可保持色澤明亮度與黃色度並延長保存期限。

Banana is one of major fruit in Taiwan. Excessive production of bananas often causes price collapses and major losses to farmers. Browning is one of the problems caused by banana processing need to overcome in current banana processing industry. The purpose of this study was to add different proportions of citric acid、ascorbic acid and trehalose to manufacture low browning banana puree, and to investigate the effect of high pressure processing (HPP) and thermal treatment on the low browning banana.The results of the study show that the use of a combination of citric acid 0.5%, ascorbic acid 0.5% and trehalose 10% simultaneous application of HPP had a polyphenol oxidase (PPO) activity of 963 (U/mg protein), which was lower than that of the control group 9137 (U/mg protein). After 60°C treatment, PPO activity of 228 (U/mg protein) was lower than control group 7648 (U/mg protein), significantly decreased the PPO activity.After 75°C treatment, all of group PPO activity completely inactive. In terms of color, the L* and b* values are the highest in banana puree treated at 60 °C, and the brightness and yellowness are higher, but the total phenol and total flavonoid content were greatly reduced after heating. Overall, added citric acid, ascorbic acid and trehalose simultaneous application of high pressure processing is significant effect on reducing browning of banana puree. The banana puree was made to banana juice and added with different proportions of complex acid and HPP to inhibit browning. Though the results of sensory evaluation showed that the highest overall acceptance was in the control group, the color was poor, and the group E with 2% ascorbic acid is relatively close to the control group in each score, and the color acceptance is higher. In the future, the taste can be adjusted. Adding with 2% ascorbic acid maintains the color brightness and yellowness during storage and extends the shelf life.

王正隆。2008。‘北蕉’與‘寶島蕉’果實採收後生理及包裝對香蕉轉色及品質之影響。國立屏東科技大學農園生產系碩士論文。屏東，台灣。
王進琦、王西華。2002。食品微生物(2版)。藝軒圖書出版社。台北，台灣。
全秀華。2003。香蕉的營養成分－智慧之果。財團法人台灣癌症基金會。2019 年4 月3 日，取自：http://www.canceraway.org.tw/page.asp?IDno=339
謝廉一、劉彥彤、蔡謹如。2018。107年6月主要農作物生產預測。行政院農業委員會。台灣。
行政院衛生署。2019。台灣地區食品營養成分表。2019 年4 月3 日，取自：https://consumer.fda.gov.tw/Food/TFND.aspx?nodeID=178
呂美玲。2009。抗褐變劑對生鮮截切蘋果及蓮霧之微生物品質及色澤之影響。中興大學食品暨應用生物科技學系。台中，台灣。
李允先、徐永鑫、黃湞鈺。2013。台灣北蕉在成熟過程中之物理與生化特性。餐飲暨觀光。第 10 卷第 2 期。第 113-127 頁。台灣。
李淑英、陳昭源、趙治平。2013。台灣香蕉種原多元化與栽培管理。台灣博物季刊。第 32 卷第 2 期。第 24-33 頁。台灣。
汪多仁。1998。國內外酸味劑的現狀和展望。中國食品用化學品。中國。
何國慶、丁立孝。2007。食品酵素學。五南圖書出版股份有限公司。台北，台灣。
余文青。2008。利用Sulfolobus solfataricus ATCC 35092 之原生型與突變型麥芽寡糖苷海藻糖生成酶及麥芽寡糖苷海藻糖水解酶由澱粉生產海藻糖之研究。國立臺灣海洋大學食品科學系碩士論文。基隆，台灣。
金時俊。1992。食品添加劑－現狀、生產、性能、應用。華東化工學院出版社。上海，中國。
金利食安科技股份有限公司。2012。HPP技術優勢。2019 年4 月3 日，取自：http://www.kfs-foodtech.com/chhtmL/content.asp?cid=129&cid1=137
林耕年。1995。園產加工。復文書局。台南，台灣。
林頎生、葉瑞月。2004。感官品評應用與實作。睿煜出版社。屏東，台灣。
邱輝龍、石家宇。2013。香蕉的利用。台灣博物季刊。第33卷。第76-85頁。
侯偉。2008。酸味劑的種類及其應用。農產品加工。
施明智。2009。食物學原理 (第三版)。藝軒圖書出版社。台北。台灣。
財團法人台灣香蕉研究所。2007。台灣香蕉栽培種。
張為憲。2003。高等食品化學。華香園出版社。台北，台灣。
張佑安、陳冠仲、張翔、林信成。2014。香蕉研究現況及其廢棄物利用。元培科技大學生物科技系。中洲科技大學保健食品系。台中，台灣。
陳怡伶。2017。低褐變香蕉果肉與果皮餡料之開發。國立屏東科技大學食品科學系碩士論文。屏東，台灣。
區少梅。2003。食品感官品評學及實習。華格那企業有限公司。台中，台灣。
雷久南。1997。身心靈整體健康。琉璃光出版社。
潘瑞樴。2006。植物生理學(第一版)。藝軒圖書出版社。台北，台灣。
顏國欽、劉展冏、韓建國、劉冠汝、李嘉展、陳建元、孫芳明、蘇敏昇、馮惠萍、謝秋蘭、饒家麟、梁弘人、林聖敦、江伯源、李政達、盧更煌、周志輝。2007。食品化學。華格那企業有限公司。台中，台灣。
Aurore, G., Parfait, B., Fahrasmane, L. 2009. Bananas, raw materials for making processed food products. Trends in Food Science & Technology. 20: 78-91.
Amri, F. S. A., Hossain, M. A. 2018. Comparison of total phenols, flavonoids and antioxidant potential of local and imported ripe bananas. Egyptian Journal of Basic and Applied Sciences. 5(4): 245-251.
AOAC. 1995. Official and methods of analysis. 16th ed. Association of Official Analytical Chemists. Washington, D.C.
Baltacıoğlu, H., Bayındırlı, A., Severcan, M., Severcan, F. 2015. Effect of thermal treatment on secondary structure and conformational change of mushroom polyphenol oxidase (PPO) as food quality related enzyme: A FTIR study. Food Chemistry. 187: 263-269.
Bayindirli, A., Alpas, H., Bozoğlu, F., Hızal, M. 2006. Efficiency of high pressure treatment on inactivation of pathogenic microorganisms and enzymes in apple, orange, apricot and sour cherry juices. Food Control. 17(1): 52-58.
Bogh-Sorensen, L. 1994. Description of hurdles. Food Preservation by Combined Processes. 7-24.
Campos, C. F., Souza, P. E. A., Coelho, J. V., Gloria, M. B. A. 1996. Chemical Composition, enzyme activity and effect of enzyme inactivation on flavor quality of green coconut water. Journal of Food Processing and Preservation. 20: 487-500.
Campos, F. P., Dosualdo, G. L., Cristianini, M. 2003. Utilização da tecnologia de alta pressão no processamento de alimentos. Brazilian Journal of Food Technology. 6: 351-357.
Cano MP, Ancos B, Matallana MC, Camara M, Reglero GT, Abera J. 1997. Differences among Spanish and Latin-American banana cultivars: morphological, chemical and sensory characteristics. Food Chemistry. 59: 411-419.
Chang C. C., Yang M. H., Wen H. M., Chern, J. C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10(3): 178-182.
Chakraborty, S., Kaushik, N., Rao, P. S., Mishra, H. N. 2014. High‐pressure inactivation of enzymes: a review on its recent applications on fruit purees and juices. Comprehensive Reviews in Food Science and Food Safety. 13(4): 578-596.
Cao, S., Zheng, Y., Wang, K., Rui, H., Tang, S. 2010. Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest. Food Chemistry. 118: 641-647.
Ding, Z. S., Tian, S. P., Zheng, X. I., Zhou, Z. W., Xu, Y. 2007. Responses of reactive oxygen metabolism and quality in mango fruit to exogenous oxalic acid or salicylic acid under chilling temperature stress. Physiologia Plantarum. 130: 112-121.
Fernandez, M. V., Denoya, G. I., Jagus, R. J., Vaudagna, S. R., Agüero, M. V. 2019. Microbiological, antioxidant and physicochemical stability of a fruit and vegetable smoothie treated by high pressure processing and stored at room temperature. LWT. 105: 206-210.
Friedman, M. 1996. Food browning and its prevention: an overview. Journal of Agricultural and Food Chemistry. 44: 631-653.
Food and agriculture organization of the United Nations. 2015. Banana and plantain processing technologies. FAOSTAT.
Farkas, D. F., & Hoover, D. G. 2000. High pressure processing. Journal of Food Science. 65: 47-64.
Guerrero, S., Alzamora, S. M., Gerschenson, L. N. 1996. Optimization of a combined factors technology for preserving banana purée to minimize colour changes using the response surface methodology. Journal of Food Engineering. 28:307-322.
Galeazzi, M. A., Sgarbieri, V. C., Constantinides, S. M. 1981. Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L). Journal of Food Science, 46: 150-155.
Gawlik-Dziki, U. 2008. Effect of hydrothermal treatment on the antioxidant properties of broccoli (Brassica oleracea var. botrytis italica) florets. Food Chemistry 109: 393-401.
Huang, H., Jing G., Guo, L., Zhang, D., Yang, B., Duan, X., Ashraf, M., Jiang, Y. 2013. Effect of oxalic acid on ripening attributes of banana fruit during storage. Postharvest Biology and Technology. 84: 22-27.
Higashiyama, T. 2002. Novel functions and applications of trehalose. Pure Applied Chemistry, 74: 1263-1269.
Huang, H., Zhu, Q., Zhang, Z., Yang, B., Duan, X., Jiang, Y. 2013. Effect of oxalic acid on antibrowning of banana (Musa spp., AAA group, cv. ‘Brazil’) fruit during storage. Scientia Horticulturae. 160: 208-212.
Jiang, Y., Fu, J., Zauberman, G., Fuchs, Y. 1999. Purification of polyphenol oxidase and the browning control of litchi fruit by glutathione and citric acid. Journal of the Science of Food and Agriculture. 79(7): 950-954.
Kanaawa, K., Sakakibara, H. 2000. High content of dopamine a strong antioxidant in Cavendish banana. Journal of Agricultural and Food Chemistry. 48: 844-848.
Klim, M., Nagy, S. 1988. An improved method to determine Nonenzymatic browning in citrus juices. Journal of Agricultural and Food Chemistry. 36: 1271-1274.
Limbo, S., Piergiovanni, L. 2007. Minimally processed potatoes: Part 2. Effects of high oxygen partial pressures in combination with ascorbic and citric acid on loss of some quality traits. Postharvest Biology and Technology. 43: 221-229.
Li, K., Weidhaas, J., Lemonakis, L., Khouryieh, H., Stone, M., Jones, L., Shen C. 2017. Microbiological quality and safety of fresh produce in West Virginia and Kentucky farmers’ markets and validation of a post-harvest washing practice with antimicrobials to inactivate Salmonella and Listeria monocytogenes. Food Control. 79: 101-108.
Leterme, P., Buldgen, A., Estrada, F., Londono, A. M. 2006. Mineral content of tropical fruits and unconventional foods of the Andes and the rain forest of Colombia. Food Chemistry. 95: 644-652.
Leistner, L. 2000. Basic aspects of food preservation by hurdle technology. International Journal of Food Microbiology. 55(1-3): 181-186.
Lim, Y. Y., Lim, T. T., Tee, J. J. 2007. Antioxidant properties of several tropical fruits: a comparative study. Food Chemistry. 103: 1003-1008.
Martinez, M. V., Whitaker, J. R. 1995. The biochemistry and control of enzymatic browning. Trends in Food Science & Technology. 6(6): 195-200.
Moshonas, M. G., Shaw, P. E. 1989. Flavor evaluation and volatile flavor constituents of stored aseptically packaged orange juice. Journal of Food Science. 54(1): 82-85.
Marshall, M. R., Kim, J., Wei, C. 2000. Enzymatic browning in fruits, vegetables and seafoods. Food and Agricultural Organization. 41:259-312.
Nijssen, L. M., Visscher, C. A., Maarse, H., Willemsens, L. C., Boelens, M. H. 1996. Volatile compounds in food. Qualitative and Quantitative Data. 7: 14-1.
Nannyonga, S., Bakalis, S., Andrews, J., Mugampoza, E., & Gkatzionis, K. 2016. Mathematical modelling of color, texture kinetics and sensory attributes characterisation of ripening bananas for waste critical point determination. Journal of Food Engineering. 190:205-210.
Oey, I., Lille, M., Van, Loey A., Hendrickx, M. 2008. Effect of high-pressure processing on colour, texture and flavour of fruit-and vegetable-based food products: a review. Trends in Food Science & Technology. 19: 320-328.
Panadare, D., Rathod, V. K. 2018. Extraction and purification of polyphenol oxidase: a review. Biocatalysis and Agricultural Biotechnology. 14: 431-437.
Pizzocaro, F., Torreggiani, D., & Gilardi, G. 1993. Inhibition of apple polyphenoloxidase (PPO) by ascorbic acid, citric acid and sodium chloride. Journal of Food Processing and Preservation. 17: 21-30.
Patras, A., Brunton, N. P., Da, Pieve S., Butler, F. 2009. Impact of high pressure processing on total antioxidant activity, phenolic, ascorbic acid, anthocyanin content and colour of strawberry and blackberry purées. Innovative Food Science & Emerging Technologies. 10: 308-313.
Qamar, S., Shaikh, A. 2018. Therapeutic potentials and compositional changes of valuable compounds from banana - A review. Trends in Food Science & Technology. 79: 1-9.
Queiroz, C., Mendes, Lopes M. L., Fialho, E., Valente-Mesquita, V. L. 2008. Polyphenol oxidase: characteristics and mechanisms of browning control. Food Reviews International. 2: 361-375.
Rickman, J. C., Barrett, D. M., Bruhn, C. M. 2007. Nutritional comparison of fresh, frozen and canned fruits and vegetables. Part 1. Vitamins C and B and phenolic compounds. Journal of the Science of Food and Agriculture. 87: 930-944.
Ren, D., Chen, P., Wang, Y., Bi, Y., Liu, H., Li, Y. 2017. Research and application on the characteristics of compound cold water soluble gel in petrifilm aerobic count plates. LWT-Food Science and Technology. 82: 335-341.

Richards, A. B., Krakowka, S., Dexter, L. B., Schmid, H., Wolterbeek, A. P. M., Waalkens-Berendsen, D. H., Shigoyuki, A., Kurimoto, M. 2002. Trehalose: A review of properties, history of use and humantolerance, and results of multiple safety studies. Food and Chemical Toxicology. 40: 871-898.
Sarang, S. S. 2008. Ohmic heating for thermal processing of low-acid foods containing solid particulates (Doctoral dissertation, The Ohio State University).
Shibamoto, T., Tang, C. S. 1990. Minor’tropical fruits–mango, papaya, passion fruit and guava In: MORTON, ID, MacLEOD, AJ Food Flavours. Part C: The flavour of fruits.
Sharma, G., Bala, R. 2002. Digital color imaging handbook. CRC press.
Sevenich, R., Rauh, C., Knorr, D. 2016. A scientific and interdisciplinary approach for high pressure processing as a future toolbox for safe and high quality products: A review. Innovative Food Science & Emerging Technologies. 38: 65-75.
Tauscher, B. 1995. Pasteurization of food by hydrostatic high pressure: chemical aspects. Zeitschrift für Lebensmittel-Untersuchung und Forschung. 200: 3-13.
Ünal, M. Ü. 2007. Properties of polyphenol oxidase from Anamur banana (Musa cavendishii). Food Chemistry. 100: 909-913.
Verma, A. K., Singh, S. B., Agarwal, A. K., Solomon, S. 2012. Influence of postharvest starge temperature, time, and invertase enzyme activity on sucrose and weight loss in sugarcane. Postharvest Biology and Technology. 73: 14-21.
Walkling-Ribeiro, M., Noci, F., Cronin, D. A., Riener, J., Lyng, J. G., Morgan, D. J. 2008. Reduction of Staphylococcusaureus and quality changes in apple juice processed by ultraviolet irradiation, pre-heating and pulsed electric fields. Journal of food engineering. 89(3): 267-273.
Wolfrom, M. L., Kashimura, N., Horton, D. 1974. Factors affecting the Maillard browning reaction between sugars and amino acids. Nonenzymic browning of dehydrated orange juice. Journal of Agricultural snd Food Chemistry. 22: 796-800.
Wiley, R. C. 1994. Preservation methods for minimally processed refrigerated fruits and vegetables. In Minimally Processed Refrigerated Fruits & Vegetables (pp. 66-134). Springer, Boston, MA.
Wall, M. M. 2006. Ascorbic acid, vitamin A, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii. Journal of Food Composition and Analysis. 19: 434-445.
Waterborg, J. H. 2009. The protein protocols handbook: The Lowry method for protein quantitation. Totowa. NJ: Humana Press
Walkling‐Ribeiro, M., Noci, F., Cronin, D. A., Lyng, J. G., & Morgan, D. J. 2008. Inactivation of Escherichia coli in a tropical fruit smoothie by a combination of heat and pulsed electric fields. Journal of food science. 73: 395-399.
Wu, B., Pan, Z., Qu, W., Wang, B., Wang, J., & Ma, H. 2014. Effect of simultaneous infrared dry-blanching and dehydration on quality characteristics of carrot slices. LWT-Food Science and Technology. 57: 90-98.
Yoruk, R., Marshall, M. R. 2003. Physicochemical properties and function of plant polyphenol oxidase: a review 1. Journal of Food Biochemistry. 27: 361-422.
Xiao, H. W., Bai, J. W., Sun, D. W., Gao, Z. J. 2014. The application of superheated steam impingement blanching (SSIB) in agricultural products processing–A review. Journal of Food Engineering. 132: 39-47.
Xu, Z., Wang, Y., Ren, P., Ni, Y., Liao, X. 2016. Quality of banana puree during storage: A comparison of high pressure processing and thermal pasteurization methods. Food and Bioprocess Technology. 9: 407-420.
Zhao, G., Zhang, R., Zhang, M. 2017. Effects of high hydrostatic pressure processing and subsequent storage on phenolic contents and antioxidant activity in fruit and vegetable products. International Journal of Food Science & Technology. 52: 3-12.