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研究生: 陳彥伃
Chen, Yen-Yu
論文名稱: 木鱉果萃取物對小鼠肝臟FL83B細胞胰島素阻抗之改善
Amelioration of Gac (Momordica cochinchinensis) Extracts on the Insulin Resistance of Mouse Liver Cell FL83B
指導教授: 吳明昌
Wu, Ming-Chang
吳美莉
Wu, Mei-Li
學位類別: 碩士
Master
系所名稱: 農學院 - 食品科學系所
Department of Food Science
畢業學年度: 108
語文別: 中文
論文頁數: 67
中文關鍵詞: 木鱉果第二型糖尿病葡萄糖攝取量
外文關鍵詞: Momordica cochinchinensis, type 2 diabetes, glucose uptake
DOI URL: http://doi.org/10.6346/NPUST202000003
相關次數: 點閱:15下載:0
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  • 中文摘要 I
    ABSTRACT II
    謝誌 III
    目錄 IV
    圖目錄 VII
    表目錄 X
    第一章 前言 1
    第二章 文獻回顧 2
    2.1. 木鱉果 2
    2.1.1 木鱉果品種與分佈 2
    2.2. 糖尿病 3
    2.2.1 糖尿病簡介 3
    2.2.2 糖尿病分類 4
    2.2.3 糖尿病各項檢驗指標 5
    2.2.4 糖尿病症狀與併發症 6
    2.3抗糖尿病藥物. 6
    2.3.1 口服降血糖藥物 6
    2.4. 胰島素 9
    2.4.1 胰島素簡介 9
    2.4.2 胰島素傳訊分子 9
    2.4.3 胰島素阻抗 14
    2.5. 肝臟中葡萄糖轉運與代謝途徑 14
    2.5.1 葡萄糖轉運蛋白 14
    2.5.2 周邊組織之葡萄糖轉運 14
    第三章 材料與方法 15
    3.1 實驗材料 15
    3.2 實驗藥品 15
    3.3 儀器設備 17
    3.4 試劑配製 18
    3.5 實驗細胞株 18
    3.6 細胞數目之計算 18
    3.6.1.細胞培養 19
    3.7 實驗架構 20
    3.8 樣品萃取 22
    3.9 木鱉果萃取物對細胞毒性試驗(MTT Assay) 22
    3.10高葡萄糖誘導細胞產生胰島素阻抗 23
    3.10.1葡萄糖擬似物(2-NBDG) 23
    3.10.2 人類胰島素溶液製備 24
    3.10.3葡萄糖添加量對細胞誘導之效益 24
    3.10.4以高葡萄糖誘導細胞產生胰島素阻抗之評估 24
    3.11胰島素阻抗細胞之2-NBDG 攝入量分析 24
    3.12西方墨點法 25
    3.12.1細胞蛋白質萃取 25
    3.12.2蛋白質標準曲線製備 25
    3.12.3聚丙烯醯安膠體與電泳法分析 25
    3.12.4轉漬與免疫雜交反應 26
    3.12.5顯影與定影 26
    3.13統計分析 26
    第四章 結果與討論 27
    4.1 木鱉果萃取物及葡萄糖誘導細胞存活率分析 27
    4.1. 1高葡萄糖對正常小鼠肝細胞細胞存活率分析 27
    4.1. 2木鱉果樣品對正常小鼠肝細胞細胞存活率分析 27
    4.2 高葡萄糖誘導FL83B細胞胰島素阻抗之評估 32
    4.3 木鱉果萃取物對具胰島素阻抗之FL83B細胞葡萄旁攝入量之影響 32
    4.3. 1木鱉果果肉各層萃取物對具胰島素阻抗之FL83B細胞葡萄糖攝入量之影響 32
    4.3. 2木鱉果假種皮各層萃取物對具胰島素阻抗之FL83B細胞葡萄糖攝入量之影響 33
    4.3. 3木鱉籽各層萃取物對具胰島素阻抗之FL83B細胞葡萄糖攝入量之影響 33
    4.4 西方墨點法 44
    4.4. 1木鱉果乙酸乙脂萃取物對胰島素阻抗FL83B細胞蛋白表現量之影響……………………………………………………………………….44
    第五章 結論 60
    第六章 參考文獻 61
    作者簡介 66
    附錄 67

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