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研究生: 古景友
Ku, Ching-Yu
論文名稱: 台灣下痢鴿子之沙門氏桿菌及大腸桿菌的調查
Investigation of Salmonella and Escherichia coli in Diarrhea pigeons in Taiwan
指導教授: 吳弘毅
Wu, Hung-Yi
學位類別: 碩士
Master
系所名稱: 獸醫學院 - 獸醫學系所
Department of Veterinary Medicine
畢業學年度: 108
語文別: 中文
論文頁數: 53
中文關鍵詞: 聚合酶鏈鎖反應鴿子沙門氏桿菌大腸桿菌
外文關鍵詞: PCR, pigeon, Salmonella, Escherichia coli
DOI URL: http://doi.org/10.6346/NPUST202000029
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  • 沙門氏桿菌(Salmonella spp.)與大腸桿菌(Escherichia coli)是鴿子體內的常在菌,若其體內有致病型的病原菌,當鴿子處在環境改變或氣候過度的變化所產生之『緊迫現象』,體內致病性病原菌乘機在鴿子體內大量的繁殖,導致鴿子出現下痢症狀,而了解鴿子的致病原因、感染來源、與病原菌繁殖力與季節的影響、不同年齡層受感染的程度等層面,將能成功控制鴿子感染傳播的重要流行病學因素。所以本研究想了解台灣地區鴿子沙門氏桿菌與大腸桿菌的感染情況,有助於沙門氏桿菌與大腸桿菌之預防與控制。本研究期間為2017年4月至2019年3月,從北部、中部和南部地區每個月收集30個泄殖腔拭子樣本或鴿糞樣本,共有720個田間樣本,同時收集其性別、年齡、季節、地區、飼養的階段、飼養功能類別(賽鴿或種鴿)、飼養環境和健康狀況等相關資訊,通過聚合酶連鎖反應(Polymerase Chain Reaction, PCR)鑑定沙門氏桿菌與大腸桿菌。結果顯示,在720隻鴿子樣本中檢出沙門氏桿菌的發生率為27.8%,大腸桿菌的發生率為56.7%。1個月以上未滿3個月之幼鴿得到沙門氏桿菌及大腸桿菌之比例最高,其次是未滿1個月之幼鴿,再者是3個月以上未滿1年的鴿子,發生率最低的是1年以上的成鴿。沙門氏桿菌與大腸桿菌的發生率皆在5至7月(夏)的比例最高,其次是2至4月(春),再次是8至10月(秋),發生率最低的是11至1月(冬)。感染沙門氏桿菌與大腸桿菌的發生率分別在台灣北、中、南地區分布情況,沙門氏桿菌與大腸桿菌發生在南部的情況最多,其次是中部,北部最少。沙門氏桿菌與大腸桿菌的發生率與在各飼養階段所占的比例,在訓練期的階段中沙門氏桿菌與大腸桿菌的發生率最高,其次是比賽期,育雛期最少。在性別區別上感染沙門氏桿菌與大腸桿菌的情況,顯示母鴿感染沙門氏桿菌與大腸桿菌的比例較公鴿高。賽鴿感染沙門氏桿菌與大腸桿菌的比例皆高於種鴿感染沙門氏桿菌與大腸桿菌的比例。

    Salmonella and Escherichia coli are common bacteria in pigeons. If there are pathogenic pathogens in the body, when the pigeons are exposed to "urgent phenomena" caused by environmental changes or excessive climate changes, pathogenic pathogens in the body take the opportunity to A large amount of breeding in pigeons leads to the symptoms of diarrhea in pigeons. Understanding the cause of the pigeons, the source of infection, the reproductive power of pathogens and the effects of seasons, and the degree of infection at different ages will successfully control the spread of pigeon infections. Important epidemiological factors. Therefore, this study wants to understand the infection status of Salmonella and Escherichia coli in Taiwan, and it is helpful for the prevention and control of Salmonella and Escherichia coli. During the study period, from April 2017 to March 2019, 30 cloaca swab samples or pigeon dung samples were collected from the northern, central and southern regions each month. There were 720 field samples and their gender, age and season were collected. Information on Salmonella and Escherichia coli by Polymerase Chain Reaction(PCR), information on areas, feeding stages, feeding function categories (race or breeders), feeding environment and health status. The results showed that the incidence of Salmonella in 720 pigeon samples was 27.8%, and the incidence of Escherichia coli was 56.7%. Young pigeons with more than 1 month and less than 3 months have the highest proportion of Salmonella and Escherichia coli, followed by young birds less than 1 month, and pigeons less than 3 months old, infection rate The lowest is an old bird of more than one year. The incidence of Salmonella and Escherichia coli was highest in May to July (summer), followed by February to April (spring), and again from August to October (autumn). The lowest incidence was November to January(winter). The incidence of Salmonella infection and Escherichia coli was distributed in the northern, central and southern parts of Taiwan. Salmonella and Escherichia coli occurred most in the south, followed by the central part and the least in the north. The incidence of Salmonella and Escherichia coli and the proportion of each breeding stage, the highest infection rate of Salmonella and Escherichia coli during the training period, followed by the competition period, the least during the brooding period. Infections between Salmonella and Escherichia coli in terms of gender differences indicate that the ratio of Salmonella to Escherichia coli in hens is higher than that of cocks. The ratio of Salmonella to Escherichia coli in the pigeons is higher than that of the pigeons infected with Salmonella and Escherichia coli.

    目錄
    中文摘要 I
    Abstract III
    謝誌 V
    目錄 VI
    圖表目錄 X
    第1章 前言 1
    第2章 文獻回顧 4
    2.1 賽鴿活動簡介 4
    2.2 細菌性腸炎 5
    2.3 沙門氏桿菌 5
    2.3.1 沙門氏桿菌簡介 5
    2.3.2 沙門氏桿菌流行病學 6
    2.3.3 沙門氏桿菌臨床症狀 7
    2.3.4 沙門氏桿菌傳播方式 7
    2.3.5 沙門氏桿菌治療與預防控制 8
    2.4 大腸桿菌 8
    2.4.1 大腸桿菌簡介 8
    2.4.2 大腸桿菌流行病學 9
    2.4.3 大腸桿菌臨床症狀 10
    2.4.4 大腸桿菌傳播方式 13
    2.4.5 大腸桿菌治療與預防控制 13
    第3章 材料與方法 15
    3.1 實驗架構 15
    3.2 病材收集 15
    3.3 臨床問診 16
    3.4 分子生物學檢測 16
    3.4.1 核酸萃取 16
    3.4.2 聚合酶鏈鎖反應之檢測 18
    3.4.3 PCR產物之純化 19
    3.4.4 重組質體及選質 20
    3.4.5 質體DNA之純化 20
    第4章 結果 22
    4.1 PCR檢測 22
    4.2 Salmonella spp. PCR敏感性試驗 22
    4.3 Escherichia coli PCR敏感性試驗 22
    4.4 台灣地區下痢鴿子之沙門氏桿菌及大腸桿菌發生率 23
    4.5 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在各年齡所占的比例 24
    4.6 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌在陽性檢出之樣本中各年齡所占比例 25
    4.7 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在各季節所占的比例 27
    4.8 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌在陽性檢出之樣本中各季節所占比例 28
    4.9 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率與在北、中、南部所占的比例 29
    4.10 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌在陽性檢出之樣本中北、中、南部所占的比例 30
    4.11 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率與在各飼養階段所占的比例 32
    4.12 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌在陽性檢出之樣本中各飼養階段所占的比例 33
    4.13 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在公鴿及母鴿所占的比例 34
    4.14 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌在陽性檢出之樣本中公鴿及母鴿所占的比例 35
    4.15 台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在賽鴿和種鴿所占的比例 37
    4.16 台灣地區下痢鴿子之沙門氏桿菌及大腸桿菌在陽性檢出之樣本中賽鴿和種鴿所占的比例 38
    4.17 結論 40
    第5章 討論 42
    參考文獻 46
    作者簡介 53

    圖表目錄
    圖 1實驗架構 15
    圖 2核酸萃取步驟 17
    圖 3聚合酶鏈鎖反應儀 18
    圖 4在電泳槽進行電泳反應 19
    圖 5透過照膠機照相獲得DNA片段位置 19
    圖6 Salmonella spp. 22
    圖 7 Escherichia coli PCR敏感性試驗 23
    圖 8下痢鴿子之沙門氏桿菌及大腸桿菌發生率 24
    圖 9 沙門氏桿菌與大腸桿菌的發生率在各年齡所占的比例 25
    圖 10 台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中各年齡所占比例 26
    圖 11台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中各年齡所占比例 26
    圖 12台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在各季節所占的比例 27
    圖 13台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中各季節所占比例 28
    圖 14台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中各季節所占比例 29
    圖 15台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率與在北、中、南部所占的比例 30
    圖 16台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中北、中、南部所占的比例 31
    圖 17台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中北、中、南部所占的比例 31
    圖 18台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率與在各飼養階段所占的比例 32
    圖 19 台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中各飼養階段所占的比例 33
    圖 20台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中各飼養階段所占的比例 34
    圖 21台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在公鴿及母鴿所占的比例 35
    圖 22 台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中公鴿及母鴿所占的比例 36
    圖 23台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中公鴿及母鴿所占的比例 37
    圖 24台灣地區下痢鴿子之沙門氏桿菌與大腸桿菌的發生率在賽鴿和種鴿所占的比例 38
    圖 25台灣地區下痢鴿子之沙門氏桿菌在陽性檢出之樣本中賽鴿和種鴿所占的比例 39
    圖 26台灣地區下痢鴿子之大腸桿菌在陽性檢出之樣本中賽鴿和種鴿所占的比例 40
    表 1特異性引子 18
    表 2聚合酶鏈鎖反應條件 19

    王淳堯 (2007) 台灣賽鴿文化「空」景之研究—以中部二鄉鎮為例。東海大學,台中市。
    古日月 (1999) 最新養鴿入門。綜合出版社,台南市。
    何郁庭、陳麗如、陳筠凡 (2018) 返家路遙遙:賽鴿是台灣之光,還是非法博弈?動物當代思潮。取自:https://opinion.udn.com/opinion/story/10673/3398693。
    吳芳姿、王明琴、陳豪勇 (2005) 台灣地區下痢型病原性大腸桿菌流行概況分析。疫情報導 21:770-788。
    呂政峰 (1999) 養鴿與賽鴿。世一書局,台南市。
    杜蔷、徐士忠、彭海凌 (1997) 禽大腸桿菌病的危害及診斷。中國家禽 9:20。
    邢淵博 (2013) 台灣賽鴿的社會學分析。南華大學,嘉義縣。
    劉仲康 (1996) 認識大腸桿菌。科學月刊 322:809-812。
    蔡裕仁、李建平、趙馨華 (1996) 台北地區飼養鴿糞便中沙門氏桿菌分離調查。中華民國獸醫學會雜誌 22:417-420。
    Baldini, M. M., J. P. Nataro and J. B. Kaper (1986) Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli. Infect Immun 52:334-336.
    Baudry, B., S. J. Savarino, P. Vial, J. B. Kaper and M. M. Levine (1990) A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 161:1249-1251.
    Brenner, F. W., R. G. Villar, F. J. Angulo, R. Tauxe and B. Swaminathan (2000) Salmonella Nomenclature. J Clin Microbiol 38:2465-2467.
    Cheong, Y., Y. Jin Kim, H. Kang, S. Choi and H. Joo Lee (2017) Label-free identification of antibiotic resistant isolates of living Escherichia coli: Pilot study. Microsc Res Tech 80:177-182.
    Chiu, C. H., L. H. Su and C. Chu (2004) Salmonella enterica serotype Choleraesuis: epidemiology, pathogenesis, clinical disease, and treatment. Clin Microbiol Rev 17:311-322.
    Chiu, C. H., T. L. Wu, L. H. Su, J. W. Liu and C. Chu (2004) Fluoroquinolone resistance in Salmonella enterica serotype Choleraesuis, Taiwan, 2000-2003. Emerg Infect Dis 10:1674-1676.
    Cohen, N. D., L. J. Martin, R. B. Simpson, D. E. Wallis and H. L. Neibergs (1996) Comparison of polymerase chain reaction and microbiological culture for detection of Salmonellae in equine feces and environmental samples. Am J Vet Res 57:780-786.
    Cohen, N. D., D. E. Wallis, H. L. Neibergs, A. P. McElroy, E. D. McGruder, J. R. DeLoach, D. E. Corrier and B. M. Hargis (1994) Comparison of the polymerase chain reaction using genus-specific oligonucleotide primers and microbiologic culture for the detection of Salmonella in drag-swabs from poultry houses. Poult Sci 73:1276-1281.
    DuPont, H. L., S. B. Formal, R. B. Hornick, M. J. Snyder, J. P. Libonati, D. G. Sheahan, E. H. LaBrec and J. P. Kalas (1971) Pathogenesis of Escherichia coli diarrhea. N Engl J Med 285:1-9.
    Grimont, P. A. D., F. Grimont and P. Bouvet (2000) Taxonomy of the genus Salmonella, vol 1. Salmonella in domestic animals. CABI.
    Guthrie, R. K. (2017) Control of Salmonella Spread. Salmonella, 1st edn. CRC Press, Boca Raton.
    Holt, P. S. and L. H. Chaubal (1997) Detection of motility and putative synthesis of flagellar proteins in Salmonella pullorum cultures. J Clin Microbiol 35:1016-1020.
    Hornick, R. B. (1974) Jeremiah Metzger lecture: Salmonella infections-newer perspectives of an old infection. Trans Am Clin Climatol Assoc 85:164-174.
    Hudson, C. R., M. Garcia, R. K. Gast and J. J. Maurer (2001) Determination of close genetic relatedness of the major Salmonella enteritidis phage types by pulsed-field gel electrophoresis and DNA sequence analysis of several Salmonella virulence genes. Avian Dis 45:875-886.
    Hueck, C. J. (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62:379-433.
    Jay, J. M. (1992) Modern food microbiology, vol 4. Foodborne gastroenteritis caused by Salmonella, shigella, and Escherichia. Van Nostrand Reinhold, New York.
    Joya, J. E., T. Tsuji, A. V. Jacalne, M. Arita, T. Tsukamoto, T. Honda and T. Miwatani (1990) Demonstration of enterotoxigenic Escherichia coli in diarrheic broiler chicks. Eur J Epidemiol 6:88-90.
    Kalin, R., M. Karahan, M. Açık, B. Tasdemir and B. Cetinkaya (2017) Mastitisli İnek Sütlerinde Önemli Patojenlerin Direkt Tespiti için Bir Multipleks PCR Yönteminin Geliştirilmesi.
    Kampelmacher, E. H. (1983) Irradiation for control of Salmonella and other pathogens in poultry and fresh meats. Food Technol 37:117-119.
    Kampelmacher, E. H. (1983) Salmonellosis as a cause of food poisoning. Method of Prevention aimed at reducing the incidence of Salmonella and standards for harmonization of detection methods. Rev Sci Tech Off Int Eiz 2:941-958.
    Karmali, M. A., M. Petric, C. Lim, P. C. Fleming, G. S. Arbus and H. Lior (1985) The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis 151:775-782.
    Karmali, M. A., B. T. Steele, M. Petric and C. Lim (1983) Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 1:619-620.
    Khan, A. A., M. S. Nawaz, S. A. Khan and C. E. Cerniglia (2000) Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction. FEMS Microbiol Lett 182:355-360.
    Labrec, E. H., H. Schneider, T. J. Magnani and S. B. Formal (1964) Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. Journal of bacteriology 88:1503-1518.
    Levine, M. M. (1987) Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis 155:377-389.
    Makino, S., H. Kurazono, M. Chongsanguam, H. Hayashi, H. Cheun, S. Suzuki and T. Shirahata (1999) Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples. J Vet Med Sci 61:1245-1247.
    Mangiamele, P., B. Nicholson, Y. Wannemuehler, T. Seemann, C. M. Logue, G. Li, K. A. Tivendale and L. K. Nolan (2013) Complete genome sequence of the avian pathogenic Escherichia coli strain APEC O78. Genome Announc 1:e0002613.
    Nataro, J. P. and J. B. Kaper (1998) Diarrheagenic Escherichia coli. Clin Microbiol Rev 11:142-201.
    Nataro, J. P. and J. Martinez (1998) Diagnosisand Investigation of Diarrheagenic Escherichia coli. Methods Mol Med 15:387-406.
    Orskov, F., I. Orskov and J. A. Villar (1987) Cattle as reservoir of verotoxin-producing Escherichia coli O157:H7. Lancet 2:276.
    Popoff, M. Y., J. Bockemuhl and L. L. Gheesling (2003) Supplement 2001 (no. 45) to the Kauffmann-White scheme. Res Microbiol 154:173-174.
    Pourbakhsh, S. A. and J. M. Fairbrother (1994) Purification and characterization of P fimbriae from an Escherichia coli strain isolated from a septicemic turkey. FEMS Microbiology Letters 122:313-318.
    Raue, R., V. Schmidt, M. Freick, B. Reinhardt, R. Johne, L. Kamphausen, E. F. Kaleta, H. Müller and M.-E. Krautwald-Junghanns (2005) A disease complex associated with pigeon circovirus infection, young pigeon disease syndrome. Avian Pathol 34:418-425.
    Robins-Browne, R. M. (1987) Traditional enteropathogenic Escherichia coli of infantile diarrhea. Rev Infect Dis 9:28-53.
    Rowe, B. (1979) The role of Escherichia coli in gastroenteritis. Clin Gastroenterol 8:625-644.
    Sansonetti, P. J., D. J. Kopecko and S. B. Formal (1982) Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun 35:852-860.
    Savarino, S. J., A. McVeigh, J. Watson, A. Cravioto, J. Molina, P. Echeverria, M. K. Bhan, M. M. Levine and A. Fasano (1996) Enteroaggregative Escherichia coli heat-stable enterotoxin is not restricted to enteroaggregative E. coli. J Infect Dis 173:1019-1022.
    Scaletsky, I. C., M. L. Silva and L. R. Trabulsi (1984) Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 45:534-536.
    Schmidt, H., C. Knop, S. Franke, S. Aleksic, J. Heesemann and H. Karch (1995) Development of PCR for screening of enteroaggregative Escherichia coli. J Clin Microbiol 33:701-705.
    Su, L. H. and C. H. Chiu (2007) Salmonella: clinical importance and evolution of nomenclature. Chang Gung Med J 30:210-219.
    Tudor, D. C. (1991) Salmonellosis. Pigeon health and disease. Iowa State University Press, Ames.
    Tzipori, S., K. I. Wachsmuth, J. Smithers and C. Jackson (1988) Studies in gnotobiotic piglets on non-O157:H7 Escherichia coli serotypes isolated from patients with hemorrhagic colitis. Gastroenterology 94:590-597.
    Wolf, M. K. (1997) Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin Microbiol Rev 10:569-584.
    Wray, C. (1994) Mammalian salmonellosis. Handbook of zoonoses, 2nd edn. CRC Press, Boca Raton.
    Yamamoto, T., Y. Koyama, M. Matsumoto, E. Sonoda, S. Nakayama, M. Uchimura, W. Paveenkittiporn, K. Tamura, T. Yokota and P. Echeverria (1992) Localized, aggregative, and diffuse adherence to HeLa cells, plastic, and human small intestines by Escherichia coli isolated from patients with diarrhea. J Infect Dis 166:1295-1310.
    Zhang, A., X. He, Y. Meng, L. Guo, M. Long, H. Yu, B. Li, L. Fan, S. Liu, H. Wang and L. Zou (2016) Antibiotic and Disinfectant Resistance of Escherichia coli Isolated from Retail Meats in Sichuan, China. Microb Drug Resist 22:80-87.

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