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研究生: 劉宇翔
Liu, Yu-Xiang
論文名稱: 接種根瘤菌與叢枝菌根菌搭配不同量稻殼生物炭及有機質肥料對土壤性質、紅豆生育及產量之影響
Effects of Different Rates of Rice Husk Biochar and Organic Fertilizer Application on the Soil Properties and the Growth of Adzuki Bean Inoculated with Rhizobium and Arbuscular Mycorrhizal Fungi
指導教授: 王鐘和
學位類別: 碩士
Master
系所名稱: 農學院 - 農園生產系所
Department of Plant Industry
畢業學年度: 108
語文別: 中文
論文頁數: 143
中文關鍵詞: 土壤肥力土壤改良劑有益微生物土壤總體密度
外文關鍵詞: soil fertility, soil amendment, effective microorganisms, soil bulk density
DOI URL: http://doi.org/10.6346/NPUST202000056
相關次數: 點閱:58下載:11
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  • 紅豆 (Vigna angularis) 為台灣南部秋冬季裡作的重要經濟作物,隨著氣候極端變化與生育期間飽受病蟲之危害,加上生產成本高且產量較不穩定,致使有機栽培紅豆面臨許多困難。有機資材經低氧高熱裂解碳化後所產生之物質,稱為「生物炭」。生物炭的功能為改善土壤理化性質,促使植株生長較佳與提高作物產量,多孔隙之特性不但能保水與保肥,亦能提供微生物棲息地。本試驗目的為藉由稻殼生物炭作為土壤改良劑,並搭配有機質肥料用量與接種根瘤菌與菌根菌探討對土壤性質、紅豆生育及產量之影響。試驗處理為接種根瘤菌與菌根菌搭配施用不同量稻殼生物炭 (0 % 、2 % 、4 % 與8 % (w/w)) 與不同量有機質肥料 (含氮為45 kg ha-1、90 kg ha-1與180 kg ha-1)。結果顯示隨著稻殼生物炭用量增加可顯著降低土壤總體密度,並改善土壤田間含水量,提高土壤pH值、EC值、有機質、Bray-1-P、交換性鉀、鈣與鎂含量,其中以土壤交換性鉀含量提升效果較明顯。施用有機質肥料用量為180 kg ha-1顯著提高土壤理化性質,而接種根瘤菌處理則顯著提高土壤總無機態氮含量;接種菌根菌處理增加土壤磷有效性之含量。於植株乾物重以稻殼生物炭用量4 % 處理顯著高於其他稻殼生物炭處理,反之施用8 % 稻殼生物炭用量處理僅提高約6 %。有機質肥料用量180 kg ha-1之處理亦顯著提高植株乾物重,接菌處理則無顯著差異。紅豆產量與產量構成因子結果亦顯示4 % 與8 % 稻殼生物炭處理用量顯著高於對照組,總產量分別提高75 % 與53 %,但有機質肥料用量與接菌間皆無顯著差異。綜合上述,增加稻殼生物炭、有機質肥料用量與接種根瘤菌與叢枝菌根菌之應用的確能改善土壤理化性質,並以4 % 之稻殼生物炭用量搭配有機質肥料用量180 kg ha-1與單一接種菌根菌為最佳處理。

    Adzuki bean (Vigna angularis) is important cash crop produced by autumn and winter in southern Taiwan. With extreme changes in climate and plant diseases and pest control during growth, coupled with high production costs and unstable production, facing many problems in organic production of adzuki beans. Organic materials are produced by carbonization of low-oxygen and high-temperature pyrolysis, called "biochar". The function of biochar is to improve soil physical and chemical properties, promote better plant growth and increase crop yield. Porous characteristics not only retain water and fertilizer, but also provide microbial habitat. The purpose of this experiment was to use rice husk biochar as a soil amendment, combined with application rate from organic fertilizer and inoculation with rhizobium and mycorrhizal fungi on soil properties, adzuki bean plant growth and yield. The experiment treatments were to inoculate rhizobium and arbuscular mycorrhizal fungi with application rates of rice husk biochar (0 %, 2 %, 4%, and 8% (w / w)) and N application rates of organic fertilizer (45 kg ha-1, 90 kg ha-1 and 180 kg ha-1). The results showed that with the increase of rice husk biochar, it could significantly reduce the soil bulk density, improve the soil moisture content of field capacity, increase soil pH, EC, organic matter, Bray-1-P, exchangeable potassium, calcium and magnesium, the effect of increasing the exchangeable potassium content of soil was more obvious. The application of organic fertilizer at 180 kg ha-1 significantly improved soil physical and chemical properties, while inoculation with rhizobium significantly increased soil inorganic nitrogen content; inoculation with mycorrhizal bacteria increased soil phosphorus availability. The dry matter weight of adzuki bean plants with 4% rice husk biochar was significantly higher than that of other rice husk biochar treatments, whereas the use of 8 % rice husk biochar increased only about 6%. The N application of 180 kg ha-1 from organic fertilizer also significantly increased the dry weight of the plant, but there was no significant difference in the treatment of inoculation. The results of adzuki bean yield and yield components factors showed that 4% and 8% rice husk biochar treatments were significantly higher than that of the control treatment, and the total yield increased by 75% and 53% respectively, but there was no significant difference between the amount of organic fertilizer and inoculation treatments. In summary, the application of rice husk biochar, organic fertilizer and inoculation treatment with Rhizobium and arbuscular mycorrhizal fungi can improve the physical and chemical properties of the soil. The best treatment is to use 4% rice husk biochar with 180 kg ha-1 from organic fertilizer and inoculation with arbuscular mycorrhizal fungi.

    摘要 II
    Abstract III
    謝誌 V
    目錄 VI
    圖目錄 IX
    表目錄 X
    壹、 前言 1
    貳、 文獻回顧 3
    一、 紅豆簡介 3
    二、 有機栽培的起源與介紹 6
    三、 生物炭簡介 9
    四、 有機質肥料簡介 11
    五、 營養元素對作物生長的影響 15
    六、 微生物肥料之介紹 18
    (一) 根瘤菌之介紹 19
    (二) 菌根菌之介紹 20
    參、 材料與方法 23
    一、 供試土壤採集 23
    二、 生物炭製備 23
    三、 土壤培育試驗 23
    四、 試驗設計 24
    五、 樣本分析項目及方法 36
    (一) 土壤分析 36
    1. 土壤質地 36
    2. 土壤總體密度 37
    3. 土壤田間含水量 37
    4. 酸鹼值 (pH 值) 37
    5. 電導度 (EC值) 37
    6. 土壤有機質 37
    7. 土壤無機態氮 38
    8. 土壤Bray-1 磷 38
    9. 土壤交換性鉀、鈣和鎂 39
    (二) 植體分析 39
    1. 植體全磷、鉀、鈣、鎂之測定 39
    (三) 有機質肥料分析 39
    1. pH值 39
    2. EC值 39
    3. 水分含量 40
    4. 有機質含量 40
    5. 全氮、氮與氫含量 40
    6. 全磷、鉀、鈣、鎂、鐵、錳、銅與鋅測定 40
    六、 統計分析 41
    肆、 結果與討論 42
    一、 稻殼生物炭用量對培育試驗之土壤理化性質的影響 42
    (一) 稻殼生物炭用量對培育試驗之土壤pH值的影響 42
    (二) 稻殼生物炭用量對培育試驗之土壤EC值的影響 44
    二、 接種根瘤菌與叢枝菌根菌搭配不同量稻殼生物炭及有機質肥料對土壤理化性質之影響 46
    (一) 不同稻殼生物炭處理對土壤總體密度的影響 46
    (二) 不同稻殼生物炭處理對土壤田間容水量的影響 48
    (三) 不同處理對土壤pH值的影響 50
    (四) 不同處理對土壤EC值的影響 57
    (五) 不同處理對土壤有機質含量的影響 60
    (六) 不同處理對土壤有效磷含量的影響 62
    (七) 不同處理對土壤交換性鉀含量的影響 66
    (八) 不同處理對土壤交換性鈣含量的影響 69
    (九) 不同處理對土壤交換性鎂含量的影響 70
    (十) 不同處理對土壤總無機態氮含量的影響 72
    1. 無機態氮 72
    2. 硝酸態氮 73
    3. 銨態氮 74
    三、 接種根瘤菌與叢枝菌根菌搭配不同量稻殼生物炭及有機質肥料對紅豆產量及產量構成因子之影響 78
    四、 接種根瘤菌與叢枝菌根菌搭配不同量稻殼生物炭及有機質肥料對紅豆生育的影響 83
    (一) 不同處理對紅豆生育性狀的影響 83
    (二) 不同處理對植株乾物質的影響 88
    (三) 不同處理對植株營養元素的影響 93
    1. 不同處理對植株磷濃度的影響 93
    2. 不同處理對植株鉀濃度的影響 96
    3. 不同處理對植株鈣濃度的影響 101
    4. 不同處理對植株鎂濃度的影響 101
    伍、結論 110
    陸、 參考文獻 111
    作者簡介 143

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