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| 研究生： |
翁林煜 Weng Lin,Yu |
|---|---|
| 論文名稱： |
荖葉莖活性天然物(+)-bornyl p-coumarate誘導口腔癌細胞產生細胞凋亡之研究 Study of (+)-bornyl p-coumarate from Piper betle stems induced cell apoptosis on oral cancer cells |
| 指導教授： |
徐睿良
Hsu,Jue-Liang 吳裕仁 Wu,Yu-Jen |
| 學位類別： |
碩士 Master |
| 系所名稱： |
農學院 - 生物科技系 Department of Biological Science and Technology |
| 畢業學年度： | 108 |
| 語文別： | 中文 |
| 論文頁數： | 40 |
| 中文關鍵詞： | 荖葉 、口腔癌 、細胞凋亡 、(+)-bornyl p-coumarate |
| 外文關鍵詞： | Piper betle, oral cancer, apoptosis, (+)-bornyl p-coumarate |
| DOI URL： | http://doi.org/10.6346/NPUST202000067 |
| 相關次數： | 點閱：28 下載：0 |
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中文摘要 I
ABSTRACT II
謝誌 III
目錄 IV
圖目錄 VII
第1章 前言 1
1.1 研究背景 1
1.2 研究動機 1
第2章 文獻回顧 3
2.1口腔癌 3
2.2口腔癌發生區塊 3
2.3罹患口腔癌的原因 4
2.3.1香菸與口腔癌 4
2.3.2檳榔與口腔癌 4
2.3.3酒精與口腔癌 5
2.4 HPV與口腔癌 6
2.5口腔癌的診斷 6
2.6口腔癌的分期 7
2.7口腔癌的治療 7
2.8口腔癌預後 8
2.9荖葉介紹 9
2.10荖葉的物種及潛在力 10
2.11 胡椒科 (PIPERACEAE)胡椒屬 (PIPER)的萃取物 11
2.12 (+)-BORNYL P-COUMARATE 12
2.13何謂細胞凋亡 13
2.14 粒線體引發細胞凋亡的途徑 13
2.15 內質網壓力引發細胞凋亡途徑 14
第3章 材料與方法 15
3.1 實驗架構 15
3.2實驗方法 16
3.2.1 口腔癌細胞的培養 16
3.2.1.1 口腔癌細胞解凍 16
3.2.1.2 細胞繼代 16
3.2.1.3 計數細胞 17
3.2.1.4 種細胞至well孔盤中 17
3.2.2 MTT assay測試 17
3.2.3 MTT實驗 18
3.2.4細胞型態分析 18
3.2.5細胞聚落實驗 19
3.2.6免疫螢光染色 19
3.3.1 收集細胞內蛋白質 19
3.3.2 SDS-PAGE & Western blot 21
第4章 結果與討論 23
4.1結果 23
4.1.1荖葉萃取物(+)-bornyl p-coumarate對於CAL-27和Ca9-22人類口腔癌細胞細胞存活率之影響 23
4.1.2荖葉萃取物(+)-bornyl p-coumarate對於CAL-27和Ca9-22人類口腔癌細胞之型態分析 24
4.1.3荖葉萃取物(+)-bornyl p-coumarate對於CAL-27和Ca9-22人類口腔癌細胞之聚落分析 25
4.1.4荖葉萃取物(+)-bornyl p-coumarate對於CAL-27 & Ca9-22之螢光染色 27
4.1.5荖葉萃取物(+)-bornyl p-coumarate對於Bcl-2凋亡蛋白家族之影響 28
4.1.6荖葉萃取物(+)-bornyl p-coumarate對於MAPKs/ERK路徑之影響 29
4.1.7荖葉萃取物(+)-bornyl p-coumarate對於內質網(ER)凋亡路徑之影響 30
4.2討論 31
第5章 結論 33
作者簡介 40
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校外公開2025/02/04