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研究生: 賴文傑
Lai, Wen-Chieh
論文名稱: 自動對焦微觀影像擷取系統之研發
Development of Autofocus Micro Image Capture System
指導教授: 王耀男
Wang, Yao-Nan
學位類別: 碩士
Master
系所名稱: 工學院 - 車輛工程系所
Department of Vehicle Engineering
畢業學年度: 108
語文別: 中文
論文頁數: 81
中文關鍵詞: 三軸定位機電整合自動對焦
外文關鍵詞: Three-axis positioning, Mechatronics, Auto focus
DOI URL: http://doi.org/10.6346/NPUST202000343
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  • 摘要...I
    Abstract...III
    謝誌...VII
    目錄...VIII
    表目錄...IX
    圖目錄...X
    簡寫表...XIV
    符號表...XV
    第一章 緒論...1
    1.1 前言...1
    1.2 文獻回顧...2
    1.3 研究動機與目的...19
    第二章 研究方法...20
    2.1 簡介...20
    2.2 硬體系統架構...21
    2.2.1光學影像擷取架構...21
    2.2.2 觀測載台設計...33
    2.2.3 三軸電動馬達機構與電路控制設計...34
    2.2.4光源設計...37
    2.2.5二氧化碳細胞培養箱...40
    2.2.6橡膠減振材料...41
    2.3 軟體系統架構...41
    2.3.1程式運作流程...41
    2.3.2對焦搜尋方法...42
    2.3.3清晰度演算...43
    2.4設備與器材滅菌方法...46
    2.4.1高溫滅菌...46
    2.4.2紫外光滅菌...48
    2.4.3酒精滅菌...49
    2.5實驗觀察樣本...49
    2.5.1螢光粒子...49
    2.5.2小鼠巨噬細胞 (RAW 264.7)...51
    2.5.3小鼠胚胎纖維母細胞...52
    2.5.4螢光染劑 (CFSE)...52
    2.6實驗項目...54
    2.6.1影像倍率之驗證...54
    2.6.2系統對焦與三軸定位實驗...55
    2.6.3細胞生長觀測實驗...56
    第三章 結果與討論...57
    3.1影像實際放大倍率之探討...57
    3.2系統對焦與三軸機構之再現性與可行性研究探討...64
    3.3細胞之生長觀測...68
    第四章 結論與未來展望...75
    4.1結論...75
    4.2未來展望...76
    參考文獻...77

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